中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
24期
1718-1721
,共4页
三叉神经节%神经微丝蛋白质类%乙酰胆碱酯酶%射频温控热凝术
三扠神經節%神經微絲蛋白質類%乙酰膽堿酯酶%射頻溫控熱凝術
삼차신경절%신경미사단백질류%을선담감지매%사빈온공열응술
Trigeminal ganglion%Neurofilament proteins%Acetylcholinesterase%Radiofrequency thermocoagulation
目的 研究不同温度与持续时间下射频热凝对兔半月神经节的组织学影响.方法 利用R-2000B型温控热凝射频仪和外裸长50 mm,直径0.4 mm热敏电极,对80只兔半月神经节分别进行60、70和80 ℃温控热凝毁损实验,于术后第1、7、14和28天取出受损的半月神经节,HE染色阳性者,按石蜡切片热修复抗原实验程序行免疫组化检测,观察毁损后神经丝蛋白(NF200)、乙酰胆碱酯酶(ACHE)及毒蕈碱乙酰胆碱酯酶受体(M受体)表达情况.结果 毁损半月神经节HE染色阳性表现为神经节细胞退变,神经纤维分布不均匀,髓鞘脱失.免疫组化结果为:NF200阳性表现为神经节细胞胞质和神经纤维细胞胞质内见深棕黄色颗粒,ACHE阳性表现为神经节细胞胞质和神经纤维细胞胞质内见深棕黄色颗粒,M受体阳性表现为神经节细胞胞质和神经纤维细胞胞质内见棕黄色颗粒.同一温度下,射频持续时间相同时,随术后时间的增加,NF200、ACHE、M受体三项指标的阳性表达率逐渐增高;而射频持续1 min与3 min比较,三项指标阳性表达率差异均无统计学意义(均P＞0.05).射频持续时间相同时,70 ℃与60 ℃比较,三项指标阳性表达率均明显降低,差异均有统计学意义(均P<0.05);而70 ℃与80 ℃比较,三项指标阳性表达率差异均无统计学意义(均P＞0.05).结论 当射频毁损温度为70 ℃左右时,对半月神经节杀伤力最大,神经活性明显降低;这可能是半月神经节毁损的较适宜的温度.
目的 研究不同溫度與持續時間下射頻熱凝對兔半月神經節的組織學影響.方法 利用R-2000B型溫控熱凝射頻儀和外裸長50 mm,直徑0.4 mm熱敏電極,對80隻兔半月神經節分彆進行60、70和80 ℃溫控熱凝燬損實驗,于術後第1、7、14和28天取齣受損的半月神經節,HE染色暘性者,按石蠟切片熱脩複抗原實驗程序行免疫組化檢測,觀察燬損後神經絲蛋白(NF200)、乙酰膽堿酯酶(ACHE)及毒蕈堿乙酰膽堿酯酶受體(M受體)錶達情況.結果 燬損半月神經節HE染色暘性錶現為神經節細胞退變,神經纖維分佈不均勻,髓鞘脫失.免疫組化結果為:NF200暘性錶現為神經節細胞胞質和神經纖維細胞胞質內見深棕黃色顆粒,ACHE暘性錶現為神經節細胞胞質和神經纖維細胞胞質內見深棕黃色顆粒,M受體暘性錶現為神經節細胞胞質和神經纖維細胞胞質內見棕黃色顆粒.同一溫度下,射頻持續時間相同時,隨術後時間的增加,NF200、ACHE、M受體三項指標的暘性錶達率逐漸增高;而射頻持續1 min與3 min比較,三項指標暘性錶達率差異均無統計學意義(均P＞0.05).射頻持續時間相同時,70 ℃與60 ℃比較,三項指標暘性錶達率均明顯降低,差異均有統計學意義(均P<0.05);而70 ℃與80 ℃比較,三項指標暘性錶達率差異均無統計學意義(均P＞0.05).結論 噹射頻燬損溫度為70 ℃左右時,對半月神經節殺傷力最大,神經活性明顯降低;這可能是半月神經節燬損的較適宜的溫度.
목적 연구불동온도여지속시간하사빈열응대토반월신경절적조직학영향.방법 이용R-2000B형온공열응사빈의화외라장50 mm,직경0.4 mm열민전겁,대80지토반월신경절분별진행60、70화80 ℃온공열응훼손실험,우술후제1、7、14화28천취출수손적반월신경절,HE염색양성자,안석사절편열수복항원실험정서행면역조화검측,관찰훼손후신경사단백(NF200)、을선담감지매(ACHE)급독심감을선담감지매수체(M수체)표체정황.결과 훼손반월신경절HE염색양성표현위신경절세포퇴변,신경섬유분포불균균,수초탈실.면역조화결과위:NF200양성표현위신경절세포포질화신경섬유세포포질내견심종황색과립,ACHE양성표현위신경절세포포질화신경섬유세포포질내견심종황색과립,M수체양성표현위신경절세포포질화신경섬유세포포질내견종황색과립.동일온도하,사빈지속시간상동시,수술후시간적증가,NF200、ACHE、M수체삼항지표적양성표체솔축점증고;이사빈지속1 min여3 min비교,삼항지표양성표체솔차이균무통계학의의(균P＞0.05).사빈지속시간상동시,70 ℃여60 ℃비교,삼항지표양성표체솔균명현강저,차이균유통계학의의(균P<0.05);이70 ℃여80 ℃비교,삼항지표양성표체솔차이균무통계학의의(균P＞0.05).결론 당사빈훼손온도위70 ℃좌우시,대반월신경절살상력최대,신경활성명현강저;저가능시반월신경절훼손적교괄의적온도.
Objective To study the histological effects of different temperatures and durations of radiofrequency on the trigeminal ganglions in rabbits. Methods Trigeminal ganglions of 80 rabbits were treated by R-2000B RF thermocoagulation at 60, 70, 80 ℃ respectively and the thermal electrode of 50 mm long and 0.4 mm in diameter. The treaded trigeminal ganglions were removed for HE (hematoxylin & eosin) dying at Days 1, 7, 14 and 28. And the expressions of neurofilament protein (NF200), acetylcholinesterase (ACHE) and muscarinic receptors (M-receptor) were observed after an immunohistochemical detection of HE positive samples. Results The positive manifestations of HE were degeneration of trigeminal ganglionic cells, the distribution of nerve fibers and a loss of myelinic membrane. The positive features of immunohistochemistry were as follows: NF200: dark yellow-brown particles within cytoplasm of ganglionic cells and nerve fibers cells; ACHE: deep yellow-brown particles within cytoplasm of ganglion cells and nerve fibers cells; M-receptor: yellow-brown particles within cytoplasm of ganglionic cells and nerve fibers cells. The expressions of NF200, ACHE and M-receptor increased while the temperature and radio frequency time remained constant. There was no significant difference between the expression rates of NF200, ACHE, M receptor at the same temperature for 1 and 3 minutes (P＞0.05). When treated for the same time, the expressions of NF200, ACHE and M receptor decreased more significantly at 70 ℃ versus 60 ℃ (P<0.05). However, there was no significant difference between 70 ℃ and 80 ℃ (P＞0.05). Conclusion 70 ℃ may be a suitable temperature at which radiofrequency causes the greatest harm to trigeminal ganglions so that nerve activity decreases significantly.
