中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2012年
6期
351-356
,共6页
刘裕%黄贵心%高瑞%黄晓君%刘付贞%彭林平
劉裕%黃貴心%高瑞%黃曉君%劉付貞%彭林平
류유%황귀심%고서%황효군%류부정%팽림평
糖尿病,2型%骨钙素%二甲双胍
糖尿病,2型%骨鈣素%二甲雙胍
당뇨병,2형%골개소%이갑쌍고
Diabetes mellitus,type 2%Osteocalcin%Metformin
目的 探讨骨钙素对2型糖尿病大鼠胰岛β细胞功能的影响,分析二甲双胍干预下骨钙素与糖、脂及胰岛素代谢的关系.方法 将36只8周龄健康雄性SD大鼠(体重180 ～ 230 g)按随机数字表法分为正常对照组、非二甲双胍干预组和二甲双胍干预组,每组各12只.正常对照组予普通饲料喂养,其他2组予高脂饲料喂养.第8周末,非二甲双胍干预组和二甲双胍干预组腹腔注射25 μg/g链脲佐菌素,正常对照组注射25 μg/g无菌柠檬酸-柠檬酸钠溶液.第10周末行口服葡萄糖耐量试验,二甲双胍干预组予100 mg·kg-1·d-1二甲双胍灌胃4周,其他2组予同体积蒸馏水灌胃.第4、8、10、14周末取血,检测空腹血糖、空腹胰岛索、胰岛素敏感指数、甘油三酯、总胆固醇、高密度脂蛋白胆固醇及骨钙素水平.14周末处死大鼠,光镜下观察胰腺组织病理改变,采用免疫组化SP法检测胰腺组织骨钙素变化.采用单因素方差分析、LSD法和Pearson直线相关分析进行数据统计.结果 第10周末,非二甲双胍干预组大鼠成模10只,二甲双胍干预组大鼠成模9只.第8周末,非二甲双胍干预组和二甲双胍干预组体重、甘油三酯、总胆固醇和空腹胰岛素水平明显高于正常对照组(F值分别为86.04、74.25、13.12、157.65,均P＜0.01),高密度脂蛋白胆固醇和胰岛素抵抗指数低于正常对照组(F值分别为12.13、24.01,均P＜0.01).第14周末,非二甲双胍干预组和二甲双胍干预组胰腺组织骨钙素含量较正常对照组显著减少(分别为0.84±0.09、2.55 ±0.24、3.17 ±0.08,F=12.14,P＜0.05).Pearson相关分析示,胰腺骨钙素含量与空腹血糖、甘油三酯、总胆固醇呈负相关(r值分别为-0.811、-0.699、-0.733,均P＜0.05),与空腹胰岛素、胰岛素抵抗指数、高密度脂蛋白胆固醇呈正相关(r值分别为0.826、0.414、0.706,均P＜0.05).结论 骨钙素能促进2型糖尿病大鼠胰岛β细胞分泌胰岛索.二甲双胍干预后血清和胰腺骨钙素含量增加,提示其可能具有促进骨钙素分泌的功能.
目的 探討骨鈣素對2型糖尿病大鼠胰島β細胞功能的影響,分析二甲雙胍榦預下骨鈣素與糖、脂及胰島素代謝的關繫.方法 將36隻8週齡健康雄性SD大鼠(體重180 ～ 230 g)按隨機數字錶法分為正常對照組、非二甲雙胍榦預組和二甲雙胍榦預組,每組各12隻.正常對照組予普通飼料餵養,其他2組予高脂飼料餵養.第8週末,非二甲雙胍榦預組和二甲雙胍榦預組腹腔註射25 μg/g鏈脲佐菌素,正常對照組註射25 μg/g無菌檸檬痠-檸檬痠鈉溶液.第10週末行口服葡萄糖耐量試驗,二甲雙胍榦預組予100 mg·kg-1·d-1二甲雙胍灌胃4週,其他2組予同體積蒸餾水灌胃.第4、8、10、14週末取血,檢測空腹血糖、空腹胰島索、胰島素敏感指數、甘油三酯、總膽固醇、高密度脂蛋白膽固醇及骨鈣素水平.14週末處死大鼠,光鏡下觀察胰腺組織病理改變,採用免疫組化SP法檢測胰腺組織骨鈣素變化.採用單因素方差分析、LSD法和Pearson直線相關分析進行數據統計.結果 第10週末,非二甲雙胍榦預組大鼠成模10隻,二甲雙胍榦預組大鼠成模9隻.第8週末,非二甲雙胍榦預組和二甲雙胍榦預組體重、甘油三酯、總膽固醇和空腹胰島素水平明顯高于正常對照組(F值分彆為86.04、74.25、13.12、157.65,均P＜0.01),高密度脂蛋白膽固醇和胰島素牴抗指數低于正常對照組(F值分彆為12.13、24.01,均P＜0.01).第14週末,非二甲雙胍榦預組和二甲雙胍榦預組胰腺組織骨鈣素含量較正常對照組顯著減少(分彆為0.84±0.09、2.55 ±0.24、3.17 ±0.08,F=12.14,P＜0.05).Pearson相關分析示,胰腺骨鈣素含量與空腹血糖、甘油三酯、總膽固醇呈負相關(r值分彆為-0.811、-0.699、-0.733,均P＜0.05),與空腹胰島素、胰島素牴抗指數、高密度脂蛋白膽固醇呈正相關(r值分彆為0.826、0.414、0.706,均P＜0.05).結論 骨鈣素能促進2型糖尿病大鼠胰島β細胞分泌胰島索.二甲雙胍榦預後血清和胰腺骨鈣素含量增加,提示其可能具有促進骨鈣素分泌的功能.
목적 탐토골개소대2형당뇨병대서이도β세포공능적영향,분석이갑쌍고간예하골개소여당、지급이도소대사적관계.방법 장36지8주령건강웅성SD대서(체중180 ～ 230 g)안수궤수자표법분위정상대조조、비이갑쌍고간예조화이갑쌍고간예조,매조각12지.정상대조조여보통사료위양,기타2조여고지사료위양.제8주말,비이갑쌍고간예조화이갑쌍고간예조복강주사25 μg/g련뇨좌균소,정상대조조주사25 μg/g무균저몽산-저몽산납용액.제10주말행구복포도당내량시험,이갑쌍고간예조여100 mg·kg-1·d-1이갑쌍고관위4주,기타2조여동체적증류수관위.제4、8、10、14주말취혈,검측공복혈당、공복이도색、이도소민감지수、감유삼지、총담고순、고밀도지단백담고순급골개소수평.14주말처사대서,광경하관찰이선조직병리개변,채용면역조화SP법검측이선조직골개소변화.채용단인소방차분석、LSD법화Pearson직선상관분석진행수거통계.결과 제10주말,비이갑쌍고간예조대서성모10지,이갑쌍고간예조대서성모9지.제8주말,비이갑쌍고간예조화이갑쌍고간예조체중、감유삼지、총담고순화공복이도소수평명현고우정상대조조(F치분별위86.04、74.25、13.12、157.65,균P＜0.01),고밀도지단백담고순화이도소저항지수저우정상대조조(F치분별위12.13、24.01,균P＜0.01).제14주말,비이갑쌍고간예조화이갑쌍고간예조이선조직골개소함량교정상대조조현저감소(분별위0.84±0.09、2.55 ±0.24、3.17 ±0.08,F=12.14,P＜0.05).Pearson상관분석시,이선골개소함량여공복혈당、감유삼지、총담고순정부상관(r치분별위-0.811、-0.699、-0.733,균P＜0.05),여공복이도소、이도소저항지수、고밀도지단백담고순정정상관(r치분별위0.826、0.414、0.706,균P＜0.05).결론 골개소능촉진2형당뇨병대서이도β세포분비이도색.이갑쌍고간예후혈청화이선골개소함량증가,제시기가능구유촉진골개소분비적공능.
Objective To investigate the effect of osteocalcin on function of β-cell in type 2diabetic rats.and to analyze the relationship of metformin with osteocalcin,glucose,lipid and insulin metabolism Methods Thirty-six healthy Sprague-Dawlay ( SD) rats aged 8 weeks were randomly assigned to group A (n =12),B (n =12),and C (n =12).Those of group A were fed normally,while those of group B and C were fed with high-fat diet.At 8 weeks,25 μg/g streptozotocin ( STZ) was injected into abdominal cavity of rats in group B and C,and 25 μg/g sodium citrate solution was injected into rats in group A.At 10 weeks,oral glucose tolerance test ( OGTT) was conducted,and the rats of group C were treated with 25 mg.kg-l.d-l metformin for 4 weeks.At 4,8,10 and 14 weeks,serum levels of osteocalcin,fasting plasma glucose ( FPC),fasting insulin ( FINS),insulin sensitivity index ( ISI),triglyceride ( TG),total cholesterol ( TC),and high-density lipoprotein cholesterol ( HDL-C) were tested At 14 week,all the rats were killed,and the pancreatic tissue was observed under microscope.The expression of osteoealcin was detected by immunohistochemical method.One-way of variance,LSD t test and Pearson linear correlation were used for data analysis.Results At 10 weeks,19 diabetic rats were successfully established in group B and C.At 8 weeks,body weight,TG,TC,and FINS in group B and C were significantly higher than those in group A (F values were 86.04,74.25,13.12 and 157.65,respectively; all P ＜ 0.01 ),while HDL-C and ISI were lower (F values were 12.13 and 24.01,respectively; all P ＜0.01 ).At 14 weeks,in group B and C,osteocalcin in pancreas were significantly decreased (0.84 ±0.09,2.55 ±0.24 and 3.17 ±0.08,respectively; F value was 12.14,P ＜0.05).Pearson linear correlation analysis showed that pancreas osteocalcin was negatively correlated with FPG,TG and TC ( r values were - O.811,- 0.699 and - 0.733,respectively; all P ＜ O.05 ),and positively correlated with serum FINS,ISI,and HDL-C ( r values were O.826,0.414 and 0.706,respectively; all P ＜ 0.05).Conclusions Osteocalein could promote islet β-cells insulin secretion in type 2 diabetes rats.Serum and pancreas osteocalcin in group C was higher than that in group B after intervention with mefformin,which suggests that mefformin may promote the secretion of osteocalcin.
