CAJ | 학술논문

目的建立稳定、高表达红色荧光蛋白标记的裸鼠胰腺痛转移模型.方法将RFP基闪转入人胰腺癌细胞株SW1990.评价转染前后细胞生物学行为有无改变,并采用SW1990-RFP建立实验性肺转移模型和实验性肝转移模型.应用整体荧光成像系统评价各组转移模型肿瘤转移的发生.结果获得稳定,高表达RFP的单克隆细胞系SW1990-RFP,转染前后细胞生物学行为无明显改变.成功建立了裸鼠胰腺癌实验性肺转移模型和肝转移模型,并应用整体荧光成像系统检测到各脏器微小转移的发生.建模成功率均为66.7%(4/6).结论 RFP基因不改变SW1990的细胞生物学行为,本实验所建立的裸鼠胰腺癌转移模型稳定、可靠,具有较强的灵敏性及特异性.
목적 건립은정、고표체홍색형광단백표기적라서이선통전이모형.방법 장RFP기섬전입인이선암세포주SW1990.평개전염전후세포생물학행위유무개변,병채용SW1990-RFP건립실험성폐전이모형화실험성간전이모형.응용정체형광성상계통평개각조전이모형종류전이적발생.결과 획득은정,고표체RFP적단극륭세포계SW1990-RFP,전염전후세포생물학행위무명현개변.성공건립료라서이선암실험성폐전이모형화간전이모형,병응용정체형광성상계통검측도각장기미소전이적발생.건모성공솔균위66.7%(4/6).결론 RFP기인불개변SW1990적세포생물학행위,본실험소건립적라서이선암전이모형은정、가고,구유교강적령민성급특이성.
Objective To estabhsh stable high RFP-expressing nude mice metastasis models of pancreatic cancer. Methods RFP gene was transfected into human pancreatic cancer ceils SWi990. The biological behavior of the two cell lines was assessed. The experimental lung metastasis model and the ex- perimental liver metastasis model were established by SWI990-RFP. The metastasis of pancreatic cancer ceils was assayed by whole-body fluorescence imaging system. Results We obtained stable high RFP-ex-pressing clone SWI990-RFP. The biological behavior of SW1990 and SWI990-RFP were, not cler- ent. RFP experimental metastasis models of pancreatic cancer were successfully established with the suc-cess rate being 66.7%. The metastasis and micrometastasis of orgins in early stage were. detected by using whole-body fluorescence imaging system.. Conclusion The REP gene didn' t change the biological be-havior of SWI990. The RFP metastasis models of pancreatic cancer are stable and relia ble.