CAJ | 학술논문

目的观察大鼠坐骨神经不等径小间隙动脉套接吻合后神经再生的时相变化,探讨其神经再生的机制. 方法选用Wistar大鼠20只,4只作为套接动脉供体,其余16只切断其右下肢坐骨神经作为实验模型,随机分成坐骨神经等径外膜吻合组和不等径小间隙动脉套接吻合组,每组各8只,分别于术后7、14、21、28 d取材,苏木精-伊红(HE)染色,观察两组神经再生时相的变化. 结果不等径小间隙动脉套接组再生神经术后21d通过小间隙；手术后28 d,不等径小间隙动脉套接组再生的神经较近端数量增加,再生神经是成熟的,排列规整.结论近端较细小与远端较粗大的神经通过小间隙动脉套接吻合,神经再生出现放大效应,具有储备功能；小间隙动脉套接吻合是发挥神经再生储备功能的有效手段.
목적 관찰대서좌골신경불등경소간극동맥투접문합후신경재생적시상변화,탐토기신경재생적궤제. 방법 선용Wistar대서20지,4지작위투접동맥공체,기여16지절단기우하지좌골신경작위실험모형,수궤분성좌골신경등경외막문합조화불등경소간극동맥투접문합조,매조각8지,분별우술후7、14、21、28 d취재,소목정-이홍(HE)염색,관찰량조신경재생시상적변화. 결과 불등경소간극동맥투접조재생신경술후21d통과소간극；수술후28 d,불등경소간극동맥투접조재생적신경교근단수량증가,재생신경시성숙적,배렬규정.결론 근단교세소여원단교조대적신경통과소간극동맥투접문합,신경재생출현방대효응,구유저비공능；소간극동맥투접문합시발휘신경재생저비공능적유효수단.
Objective By the study of histological feature of the regenerated nerve at different time after operation,to explore the mechanism of the peripheral nerve regeneration when the distal nerve stump is repaired by a smaller proximal end with artery sleeve briding. Methods Four Wistar rats were served as the donors of arteris for bridging.Sixteen Wistar rats were randomized into 2 groups (n =8 per group).In control group,the right sciatic nerves were cut off and received epineurial suture.In experimental group,the right sciatic nerves received artery sleeve bridging in different size.HE staining was taken to observe the histological changes of the regenerated nerve at 7,14,21 and 28 days after operation. Results The new regenerated axons need about 21 days to pass the bridging gap.Twenty-eight days after operation,the number of the regenerated axons in distal segment was greater than that of the proximal in the experimental group.The regenerated axons were matured and more regular. Conclusion The functional reserve of repaired nerve is exists when the proximal end is smaller than the distal in size by means of amplification effect.And the method of artery sleeve bridging provides a better situation for functional reserve.