中华心律失常学杂志
中華心律失常學雜誌
중화심률실상학잡지
CHINESE JOURNAL OF CARDIAC ARRHYTHMIAS
2009年
5期
369-372
,共4页
汤宝鹏%甘天翊%许国军%李耀东%郭霞%木拉提·阿布提热合曼%阿布力孜·阿布都拉%霍强
湯寶鵬%甘天翊%許國軍%李耀東%郭霞%木拉提·阿佈提熱閤曼%阿佈力孜·阿佈都拉%霍彊
탕보붕%감천익%허국군%리요동%곽하%목랍제·아포제열합만%아포력자·아포도랍%곽강
心房颤动%胶原%基质金属蛋白酶%基质金属蛋白酶抑制剂
心房顫動%膠原%基質金屬蛋白酶%基質金屬蛋白酶抑製劑
심방전동%효원%기질금속단백매%기질금속단백매억제제
Atrial fibrillation%Collagen%Matrix metalloproteinases%Tissue inhibitors of metalloprotein-ases
目的 研究心脏瓣膜病慢性心房颤动(房颤)患者心房肌间质纤维化的分子生物学机制.方法心脏瓣膜病接受瓣膜置换手术患者45例,慢性房颤27例,窦性心律18例,手术中取右心耳组织,采用半定量逆转录-聚合酶链反应测定Ⅰ型胶原、Ⅲ型胶原、基质金属蛋白酶(MMPs)中胶原酶(MMP1、MMP8、MMP13)以及基质金属蛋白酶抑制剂(TIMPs)中TMP1、TMP2、TMP3、TMP4的mRNA表达水平.结果 (1)与窦性心律组比较,慢性房颤组Ⅰ型胶原、MMP13、MMP1的mRNA表达上调(P<0.05),TMP1、TMP2、TMP3的mRNA表达下调(P<0.05).(2)MMP1的mRNA表达与Ⅰ型胶原的mRNA表达、左心房内径呈正相关,与TMP1的mRNA表达呈负相关.MMP13的mRNA表达与Ⅰ型胶原的mRNA表达、左心房内径呈正相关,与TMP3的mRNA表达呈负相关.结论 慢性房颤患者心房肌组织中MMP1/TMP1以及MMP13/TMP3的基因转录表达水平失衡引起Ⅰ型胶原转录水平的改变,可能是慢性房颤患者心房肌间质纤维化的分子基础.
目的 研究心髒瓣膜病慢性心房顫動(房顫)患者心房肌間質纖維化的分子生物學機製.方法心髒瓣膜病接受瓣膜置換手術患者45例,慢性房顫27例,竇性心律18例,手術中取右心耳組織,採用半定量逆轉錄-聚閤酶鏈反應測定Ⅰ型膠原、Ⅲ型膠原、基質金屬蛋白酶(MMPs)中膠原酶(MMP1、MMP8、MMP13)以及基質金屬蛋白酶抑製劑(TIMPs)中TMP1、TMP2、TMP3、TMP4的mRNA錶達水平.結果 (1)與竇性心律組比較,慢性房顫組Ⅰ型膠原、MMP13、MMP1的mRNA錶達上調(P<0.05),TMP1、TMP2、TMP3的mRNA錶達下調(P<0.05).(2)MMP1的mRNA錶達與Ⅰ型膠原的mRNA錶達、左心房內徑呈正相關,與TMP1的mRNA錶達呈負相關.MMP13的mRNA錶達與Ⅰ型膠原的mRNA錶達、左心房內徑呈正相關,與TMP3的mRNA錶達呈負相關.結論 慢性房顫患者心房肌組織中MMP1/TMP1以及MMP13/TMP3的基因轉錄錶達水平失衡引起Ⅰ型膠原轉錄水平的改變,可能是慢性房顫患者心房肌間質纖維化的分子基礎.
목적 연구심장판막병만성심방전동(방전)환자심방기간질섬유화적분자생물학궤제.방법심장판막병접수판막치환수술환자45례,만성방전27례,두성심률18례,수술중취우심이조직,채용반정량역전록-취합매련반응측정Ⅰ형효원、Ⅲ형효원、기질금속단백매(MMPs)중효원매(MMP1、MMP8、MMP13)이급기질금속단백매억제제(TIMPs)중TMP1、TMP2、TMP3、TMP4적mRNA표체수평.결과 (1)여두성심률조비교,만성방전조Ⅰ형효원、MMP13、MMP1적mRNA표체상조(P<0.05),TMP1、TMP2、TMP3적mRNA표체하조(P<0.05).(2)MMP1적mRNA표체여Ⅰ형효원적mRNA표체、좌심방내경정정상관,여TMP1적mRNA표체정부상관.MMP13적mRNA표체여Ⅰ형효원적mRNA표체、좌심방내경정정상관,여TMP3적mRNA표체정부상관.결론 만성방전환자심방기조직중MMP1/TMP1이급MMP13/TMP3적기인전록표체수평실형인기Ⅰ형효원전록수평적개변,가능시만성방전환자심방기간질섬유화적분자기출.
Objective To evaluate the molecular mechanisms of atrial interstitial fibrosis in patients with chronic atrial fibrillation(AF). Methods The right atrial appendages were obtained from 45 patients with valvular heart disease who underwent heart valvular replacement surgery. 18 patients were in sinus rhythm,27 patients were in chronic atrial fibrillation. The mRNA level of collagen type Ⅰ, collagen type Ⅲ, MMP1, MMP8, MMP13, TMP1, TMP2, TMP3, TMP4 were measured by semi-quantitative reverse transcription-polymer-ase chain reaction (RT-PCR). Results (1) Compared with the sinus rhythm group, in chronic AF group, the mRNA of collagen type Ⅰ, MMP1, and MMP13 increased (P<0.05),while the mRNA of TMP1, TMP2, TMP3 significantly decreased (P<0.05). (2)The mRNA level of MMPI was significantly correlated with the mRNA level of collagen type Ⅰ and left atial dimension. The mRNA level of MMP1 was negatively correlated with the mRNA level of TMP1. The mRNA level of MMP13 was significantly correlated with the mRNA level of collagen type Ⅰ and left atial dimension. The mRNA level of MMP13 was negatively correlated with the mRNA level of TMP3. Conclusion The increased level of collagen type Ⅰ associated with selective upregulation of MMP1,13 and downregulation of TMP1,3 gcne expression in atrium might be the molecular basis of atrial interstitial fibro-sis in patients with chronic atrial fibrillation.
