CAJ | 학술논문

目的探讨家兔脊髓空洞症模型脊髓基质金属蛋白酶(MMP)-9的表达变化与脊髓空洞前状态中脊髓水肿形成的关系,评价强力霉素对脊髓空洞前状态水肿形成的抑制作用.方法中国白兔96只,随机分为4组:Kaolin组、强力霉素组、生理盐水组、假手术组各24只.家兔在麻醉后,行经皮枕大池穿刺术,Kaolin组和强力霉素组动物注入37 ℃ 25%Kaolin悬浊液0.6 ml;依同样方法生理盐水组动物仅注入37℃生理盐水0.6 ml;强力霉素组动物给予强力霉素25 mg·kg-1·d-1胃管内注入,持续14 d;假手术组只做枕大池假穿刺术,既不注射Kaolin也不注入生理盐水.在第1、3、7、14天,各时间点随机抽取Kaolin组、强力霉素组、生理盐水组、假手术组动物各6只处死后,取出颈髓,应用干湿法测定脊髓空洞前状态中脊髓含水量,应用免疫印迹和免疫组织化学方法测定脊髓中MMP-9表达含量.结果各时间点Kaolin组动物颈髓组织中MMP-9表达和脊髓含水量与生理盐水组和假手术组相比明显增高(P＜0.01);强力霉素治疗组与Kaolin组相比第1天差异无统计学意义(P＞0.05);余各时间点动物脊髓含水量及颈髓组织中MMP-9表达与Kaolin组比较有明显降低(P＜0.01).结论在实验性家兔脊髓空洞症模型中,脊髓MMP-9高表达在脊髓空洞前状态水肿形成中起重要作用,强力霉素具有临床应用治疗脊髓空洞前状态的可能性.
목적 탐토가토척수공동증모형척수기질금속단백매(MMP)-9적표체변화여척수공동전상태중척수수종형성적관계,평개강력매소대척수공동전상태수종형성적억제작용.방법 중국백토96지,수궤분위4조:Kaolin조、강력매소조、생리염수조、가수술조각24지.가토재마취후,행경피침대지천자술,Kaolin조화강력매소조동물주입37 ℃ 25%Kaolin현탁액0.6 ml;의동양방법생리염수조동물부주입37℃생리염수0.6 ml;강력매소조동물급여강력매소25 mg·kg-1·d-1위관내주입,지속14 d;가수술조지주침대지가천자술,기불주사Kaolin야불주입생리염수.재제1、3、7、14천,각시간점수궤추취Kaolin조、강력매소조、생리염수조、가수술조동물각6지처사후,취출경수,응용간습법측정척수공동전상태중척수함수량,응용면역인적화면역조직화학방법측정척수중MMP-9표체함량.결과 각시간점Kaolin조동물경수조직중MMP-9표체화척수함수량여생리염수조화가수술조상비명현증고(P＜0.01);강력매소치료조여Kaolin조상비제1천차이무통계학의의(P＞0.05);여각시간점동물척수함수량급경수조직중MMP-9표체여Kaolin조비교유명현강저(P＜0.01).결론 재실험성가토척수공동증모형중,척수MMP-9고표체재척수공동전상태수종형성중기중요작용,강력매소구유림상응용치료척수공동전상태적가능성.
Objective To investigate the relationship between the expression of MMP-9 and the formation of spinal cord edema in presyrinx state of experimental syringomyelia in rabbits and evaluate the inhibitory function of doxycline in the formation of spinal cord edema in presyrinx state. Methods A total of 96 Chinese white rabbits were divided randomly into four groups:kaolin group (n =24), doxycline treatment group (n = 24 ), physiological saline group ( n = 24 ) and control group ( n = 24 ) . Under ketamine anesthesia, 0. 6 ml of 25% kaolin solution was injected into the cisterna magna in all rabbits of kaolin and doxycline groups while 0. 6 ml of 37 ℃ physiological saline into the cistern in those of saline group;doxycline (25 mg· kg-1 · d-1 ) was applied post-operatively for doxycline group. At Days 1, 3, 7 and 14 after kaolin injection, 6 rabbits of all four groups were selected randomly for sacrificing. Water content of spinal cord could be obtained. The expression of MMP-9 activity was measured by Western blot and imumunohistochemistry. Results At any time point, the water content of spinal cord and the expression of MMP-9 in kaolin group improved obviously more than those of saline and control groups(P ＜0. 01 ). At Day 1, there was no marked difference in the water content of spinal cord and the expression of MMP-9 between doxycline and kaolin groups ( P ＞ 0. 05 ). At other time pionts, the water content of spinal cord and the expression of MMP-9 in doxycline group markedly decreased than those of kaolin group (P＜0. 01).Conclusion In the model of experimental syringomyelia, MMP-9 plays an important role in causing edema in presyrinx state. Thus doxycline may be used to prevent and treat syringomyelia.