CAJ | 학술논문

利用cDNA减法杂交、差异杂交筛选和RACE等技术,从水稻(Oryza sativa L. ssp. japonica)中克隆了一个新的绒毡层特异性cDNA,其编码基因被命名为RA39.该cDNA长1 013 bp, 编码由298个氨基酸残基组成的多肽.RA39是一个单拷贝基因,在绒毡层细胞中特异性表达,在小孢子母细胞减数分裂期的绒毡层细胞中有较高的表达活性.用PSORT和PPSEARCH软件进行的结构分析揭示出RA39蛋白的N端是一个由17个氨基酸残基组成的信号肽,该蛋白包含一个跨膜区和一个胞质尾区两个主要结构域以及多个蛋白激酶的磷酸化位点.
이용cDNA감법잡교、차이잡교사선화RACE등기술,종수도(Oryza sativa L. ssp. japonica)중극륭료일개신적융전층특이성cDNA,기편마기인피명명위RA39.해cDNA장1 013 bp, 편마유298개안기산잔기조성적다태.RA39시일개단고패기인,재융전층세포중특이성표체,재소포자모세포감수분렬기적융전층세포중유교고적표체활성.용PSORT화PPSEARCH연건진행적결구분석게시출RA39단백적N단시일개유17개안기산잔기조성적신호태,해단백포함일개과막구화일개포질미구량개주요결구역이급다개단백격매적린산화위점.
A novel tapetum-specific cDNA clone of rice, its corresponding gene designed as RA39, is isolated by RNA subtractive hybridization, differential screening and rapid amplification of cDNA ends. The RA39 cDNA is 1 013 bp in length with an open reading frame encoding 298 amino acid residues. mRNA in situ hybridization reveals that RA39 is a tapetum-specific gene, and highly expressed at the meiosis stage of pollen mother cells. The deduced protein contains a signal peptide, a transmembrane region and a cytoplasmic tail, and is predicted to localize in endoplasmic reticulum by PSORT program. This cDNA sequence did not show significant homology to any known sequences in Genbank database. RA39 is the first gene identified to be expressed specifically in tapetal cells at the meiosis stage of pollen mother cells from cereals.