CAJ | 학술논문

目的观察2型糖尿病(T2DM)外周血内皮祖细胞(endothelial progenitor cell,EPC)在体外的分化增殖能力及影响因素.方法取20例T2DM无并发症患者、19例T2DM合并血管并发症患者和21例对照人群外周血EPC培养,观察细胞形态学变化并计数,用流式细胞仪检测贴壁细胞的特异性标志.结果糖尿病血管并发症组培养获得的EPC和EPC集落低于糖尿病无并发症组(P＜0.05)和对照组(P＜0.01).EPC数量与SBP及FPG呈负相关(R=-0.266,P＜0.05;R=-0.619,P＜0.01),糖尿病人EPC集落生成数量与HbA1c水平呈负相关(R=-0.749,P＜0.05),与糖尿病病程年呈负相关(R=-0.406,P＜0.01). 结论FPG和SBP与EPC的增殖分化有关,T2DM外周血EPC数目减少,与HbA1c、SBP及病程相关.
목적관찰2형당뇨병(T2DM)외주혈내피조세포(endothelial progenitor cell,EPC)재체외적분화증식능력급영향인소.방법취20례T2DM무병발증환자、19례T2DM합병혈관병발증환자화21례대조인군외주혈EPC배양,관찰세포형태학변화병계수,용류식세포의검측첩벽세포적특이성표지.결과당뇨병혈관병발증조배양획득적EPC화EPC집락저우당뇨병무병발증조(P＜0.05)화대조조(P＜0.01).EPC수량여SBP급FPG정부상관(R=-0.266,P＜0.05;R=-0.619,P＜0.01),당뇨병인EPC집락생성수량여HbA1c수평정부상관(R=-0.749,P＜0.05),여당뇨병병정년정부상관(R=-0.406,P＜0.01). 결론FPG화SBP여EPC적증식분화유관,T2DM외주혈EPC수목감소,여HbA1c、SBP급병정상관.
Objective To isolate and identify human endothelial progenitor cell(EPC) from peripheral blood of type 2 diabetic patients and investigate the capability of differentiation and proliferation of EPC. Methods Mononuclear cells isolated from human peripheral blood of type 2 diabetic patients (diabetics without complication,n=20;diabetics with angiopathy,n=19) and control subjects (n=21) were cultured.The appearing time and the number of cell clusters and spindle-shaped cells were observed respectively.Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelial-specific markers.Fluorescence-activated cell sorting (FACS) detection of EPC was performed on attached spindle cells after 7 days. Results The number of EPC and EPC-bearing clusters in diabetic vascular complication group were significantly fewer than those of nondiabetic control subjects and diabetics without complication (P<0.01).The number of clusters were inversely correlated with HbA1c levels (R=-0.749,P<0.05) and the course of diabetes(R=-0.406,P<0.01). Conclusions There are endothelial progenitor cells in human peripheral blood, which can differentiate into endothelial cells.The dysfunction of EPC in diabetics is significantly correlated with the HbA1c levels and the course of diabetes.