CAJ | 학술논문

采用树脂石蜡(CP)组织包埋连续切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶(SP)染色法,首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究,分析了三种甲虫脑组织结构特征,初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式.结果显示,分类地位相近的三种甲虫脑结构大小相差悬殊:小青花金龟脑结构相对发达,蕈形体结构显著,具有发达的冠和叶,嗅叶发达;马铃薯瓢虫和榆紫叶甲的冠极度退化,嗅叶相对不发达;但榆紫叶甲具有十分发达的网膜后纤维和围咽神经.5-HT阳性纤维在三种甲虫的所有神经纤维网中几乎都有分布,且反应模式相似,但各神经纤维网特别是神经节层染色强度差异显著.5-HT阳性胞体有成群分布现象,且在三种甲虫脑中的空间分布位置基本一致.结果表明,三种甲虫可能由于长期生活习性的不同,在进化压力作用下产生了适应辐射,榆紫叶甲的分类地位可能相对较为低等.不同昆虫脑内5-HT阳性反应产物分布模式的相似性表明,5-HT在不同昆虫中极有可能发挥着相似的生理功能,而且5-HT可能参与调节饮食行为及视觉系统的敏感度.
채용수지석사(CP)조직포매련속절편기술,결합면역조화련매균항생물소단백-과양화물매(SP)염색법,수차대삼충갑충(유자협갑、소청화금구、마령서표충)적뇌진행료상세관찰연구,분석료삼충갑충뇌조직결구특정,초보구건료삼충갑충뇌내5-HT항원계통면역조화반응모식.결과현시,분류지위상근적삼충갑충뇌결구대소상차현수:소청화금구뇌결구상대발체,심형체결구현저,구유발체적관화협,후협발체;마령서표충화유자협갑적관겁도퇴화,후협상대불발체;단유자협갑구유십분발체적망막후섬유화위인신경.5-HT양성섬유재삼충갑충적소유신경섬유망중궤호도유분포,차반응모식상사,단각신경섬유망특별시신경절층염색강도차이현저.5-HT양성포체유성군분포현상,차재삼충갑충뇌중적공간분포위치기본일치.결과표명,삼충갑충가능유우장기생활습성적불동,재진화압력작용하산생료괄응복사,유자협갑적분류지위가능상대교위저등.불동곤충뇌내5-HT양성반응산물분포모식적상사성표명,5-HT재불동곤충중겁유가능발휘착상사적생리공능,이차5-HT가능삼여조절음식행위급시각계통적민감도.
The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size. Mushroom bodies and antennal lobes in Oxycetonia jucunda were conspicuous. Calyces and lobes of the mushroom bodies.were much developed. In contrast, calyces of Ambrostoma quadriimpressum and Henosepilachna vigintioctomaculata were extremely undeveloped. However, the postretinal fibres and circumpharyngeal nerves of Ambrostoma quadriimpressum were highly developed. In the three beetles, 5-HT immunoreactivity was present in all neuropils of the brain and the suboesophageal ganglion. The pattern of 5-HT immunoreactivity and the localization of immunoreactive somata which often clustered into groups were similar among these beetles, while the immunoreactivity intensity was distinct, especially in the lamina. The results suggest that the three beetles have given rise to adaptive radiation under the evolutionary pressure because of the long-term different life styles and living environments in which the taxonomic status of Ambrostoma quadriimpressum is relatively low. The similarity of the pattern of 5-HT immunoreactivity and localization of some positive somata among the three beetles raise the possibility that 5-HT seemes to serve similar physiological function in different insects. Furthermore, 5-HT might be involved in modulating the ingestion by regulating muscular activity and visual sensitivity.