环境工程学报
環境工程學報
배경공정학보
CHINESE JOURNAL OF ENVIRONMENTAL ENGINEERING
2009年
12期
2219-2222
,共4页
1,4-二氯苯%降解菌分离%降解特性
1,4-二氯苯%降解菌分離%降解特性
1,4-이록분%강해균분리%강해특성
dichlorobenzene%degradation strain%isolation%degradation characterization
从某污水处理曝气池的活性污泥中分离出一株能够以1,4-二氯苯为唯一碳源和能源生长的菌株DEB.1,通过形态特征和生理生化试验初步鉴定为黄杆菌属(Flavobacterium sp.).实验结果表明,该菌株最适降解温度为32℃、最适降解pH为7.8,24 h对100 mg/L的1,4-二氯苯的降解率达94.5%.菌株DEB.1的降解谱较广,对5种氯苯类物质具有较高的降解率.并进一步研究了DEB-1的1,4一二氯苯降解酶粗酶液的性质,其最适反应温度和pH分别为30℃和8.5.对处理含氯代芳香化合物的有机废水具有一定的意义.
從某汙水處理曝氣池的活性汙泥中分離齣一株能夠以1,4-二氯苯為唯一碳源和能源生長的菌株DEB.1,通過形態特徵和生理生化試驗初步鑒定為黃桿菌屬(Flavobacterium sp.).實驗結果錶明,該菌株最適降解溫度為32℃、最適降解pH為7.8,24 h對100 mg/L的1,4-二氯苯的降解率達94.5%.菌株DEB.1的降解譜較廣,對5種氯苯類物質具有較高的降解率.併進一步研究瞭DEB-1的1,4一二氯苯降解酶粗酶液的性質,其最適反應溫度和pH分彆為30℃和8.5.對處理含氯代芳香化閤物的有機廢水具有一定的意義.
종모오수처리폭기지적활성오니중분리출일주능구이1,4-이록분위유일탄원화능원생장적균주DEB.1,통과형태특정화생리생화시험초보감정위황간균속(Flavobacterium sp.).실험결과표명,해균주최괄강해온도위32℃、최괄강해pH위7.8,24 h대100 mg/L적1,4-이록분적강해솔체94.5%.균주DEB.1적강해보교엄,대5충록분류물질구유교고적강해솔.병진일보연구료DEB-1적1,4일이록분강해매조매액적성질,기최괄반응온도화pH분별위30℃화8.5.대처리함록대방향화합물적유궤폐수구유일정적의의.
A bacterial strain named DEB-1 that degraded dichlorobenzene as sole carbon and energy sources was isolated from activated sludge of a wastewater treatment plant. It was identified as Flavobacteriurn sp.according to its morphology and biochemical properties. The results showed that the optimal degradation tempera-ture of DEB-1 was 32 ℃,the optimal degradation pH value was 7.8,the degradation rate reached to 94.5% with-in 24 hours when the concentration of dichlorobenzene was 100 mg/L. The degradation spectrum of strain DEB-1 was broad and it had higher degradation for five chlorobenzenes. Further study on the characterization of strain DEB-1 degrading crude enzyme showed that the optimal reaction temperature and pH was 30 ℃ and 8.5 respec-tively. It could be useful for treating chlorinated-aromatic-containing organic wastewater.