四川大学学报(医学版)
四川大學學報(醫學版)
사천대학학보(의학판)
JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES
2010年
1期
132-136
,共5页
徐刚%王槐富%何刚%徐钢
徐剛%王槐富%何剛%徐鋼
서강%왕괴부%하강%서강
鼻腔%非霍奇金淋巴瘤%预后%P53蛋白%P21蛋白
鼻腔%非霍奇金淋巴瘤%預後%P53蛋白%P21蛋白
비강%비곽기금림파류%예후%P53단백%P21단백
Nasal cavity%Non-Hodgkin's lymphoma%Prognosis%P53 protein%P21 protein
目的 探讨P53、P21蛋白在鼻腔NK/T细胞淋巴瘤(NKTL)中的表达及其与细胞增殖、凋亡、临床分期和预后的关系.方法 收集62例NKTL患者的临床病理资料,并进行随访.取62例NKTL组织构建组织芯片,应用免疫组化方法 检测P53、P21、Ki67蛋白的表达,TUNEL法原位凋亡检测,计算增殖指数(PI)和凋亡指数(AI).结果 P53、P21蛋白在NKTL中表达的阳性率分别为79.03%、58.06%.P53和P21在Ann Arbor Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者中表达的阳性率分别为69.57%、75.00%、86.67%、100.00%和47.83%、56.25%、60.00%、87.50%,随着肿瘤进展,二者表达阳性率逐渐升高,差异具有统计学意义(P＜0.05).P53和P21二者表达具有明显的正相关性(r=0.467,P＜0.05),其阴性表达组预后好于阳性表达组(P＜0.05);P53蛋白表达是独立预后因素.随着P53、P21蛋白表达强度的增加,PI亦逐渐升高,它们均与PI呈正相关(r=0.177,0.184,P＜0.05),而与AI无关联性(P＞0.05).结论 P53、P21蛋白表达与NKTL的发生、发展密切相关,联合检测Ki67表达,可作为评估该病患者肿瘤细胞增殖、侵袭性等生物学行为的良好指标,为临床治疗及预后评价提供依据.
目的 探討P53、P21蛋白在鼻腔NK/T細胞淋巴瘤(NKTL)中的錶達及其與細胞增殖、凋亡、臨床分期和預後的關繫.方法 收集62例NKTL患者的臨床病理資料,併進行隨訪.取62例NKTL組織構建組織芯片,應用免疫組化方法 檢測P53、P21、Ki67蛋白的錶達,TUNEL法原位凋亡檢測,計算增殖指數(PI)和凋亡指數(AI).結果 P53、P21蛋白在NKTL中錶達的暘性率分彆為79.03%、58.06%.P53和P21在Ann Arbor Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者中錶達的暘性率分彆為69.57%、75.00%、86.67%、100.00%和47.83%、56.25%、60.00%、87.50%,隨著腫瘤進展,二者錶達暘性率逐漸升高,差異具有統計學意義(P＜0.05).P53和P21二者錶達具有明顯的正相關性(r=0.467,P＜0.05),其陰性錶達組預後好于暘性錶達組(P＜0.05);P53蛋白錶達是獨立預後因素.隨著P53、P21蛋白錶達彊度的增加,PI亦逐漸升高,它們均與PI呈正相關(r=0.177,0.184,P＜0.05),而與AI無關聯性(P＞0.05).結論 P53、P21蛋白錶達與NKTL的髮生、髮展密切相關,聯閤檢測Ki67錶達,可作為評估該病患者腫瘤細胞增殖、侵襲性等生物學行為的良好指標,為臨床治療及預後評價提供依據.
목적 탐토P53、P21단백재비강NK/T세포림파류(NKTL)중적표체급기여세포증식、조망、림상분기화예후적관계.방법 수집62례NKTL환자적림상병리자료,병진행수방.취62례NKTL조직구건조직심편,응용면역조화방법 검측P53、P21、Ki67단백적표체,TUNEL법원위조망검측,계산증식지수(PI)화조망지수(AI).결과 P53、P21단백재NKTL중표체적양성솔분별위79.03%、58.06%.P53화P21재Ann Arbor Ⅰ、Ⅱ、Ⅲ、Ⅳ기환자중표체적양성솔분별위69.57%、75.00%、86.67%、100.00%화47.83%、56.25%、60.00%、87.50%,수착종류진전,이자표체양성솔축점승고,차이구유통계학의의(P＜0.05).P53화P21이자표체구유명현적정상관성(r=0.467,P＜0.05),기음성표체조예후호우양성표체조(P＜0.05);P53단백표체시독립예후인소.수착P53、P21단백표체강도적증가,PI역축점승고,타문균여PI정정상관(r=0.177,0.184,P＜0.05),이여AI무관련성(P＞0.05).결론 P53、P21단백표체여NKTL적발생、발전밀절상관,연합검측Ki67표체,가작위평고해병환자종류세포증식、침습성등생물학행위적량호지표,위림상치료급예후평개제공의거.
Objective To investigate the significance of P53 and P21 expressions in nasal NK/T-cell lymphoma(NKTL)and their relationships with the clinical stage,prognosis,proliferation and apoptosis of tumor cells.Methods The clinicopathologic and follow-up data was collected from 62 patients with NKTL proven by pathological examination..Sixty-two cases of NKTL were examined for P53,P21 and Ki67 proteins with tissue microarray technique and immunohistochemistry.TUNEL method was used to detect apoptosis(apoptosis index,AI).The proliferation index(PI)was determined by the expression of Ki67 proteins.Results The positive rates of P53 and P21 protein expression in NKTL were 79.03%and 58.06%respectively.The positive rates of P53 in Ann Arbor stage Ⅰ,Ⅱ、Ⅲ and Ⅳ NKTL were 69.57%,75.00%,86.67%and 100.00%respectively,while those of P21 were 47.83%,56.25%,60.00%and 87.50%.With the progression of tumor,the positive expression rates of P53 and P21 proteins gradually increased.And there were significant differences statistically between the expressions of P53/P21 and Ann Arbor stage(P＜0.05).The expression of P53 was positively correlated with the expression of P21(r=0.467,P＜0.05).The prognosis of P53 and P21 positive expression group was worse than that of negative expression group(P＜0.05).The expression of P53 protein was an independent prognostic factor.The intensities of P53 and P21 expressions were positively correlated with PI(r=0.177,0.184,P＜0.05),while not correlated with AI(P＞0.05).Conclusion The expressions of P53,P21 and Ki67 proteins are closely related with the pathogenesis and progression of NKTL.Combined detection of P53,P21 and Ki67 could be better to judge the biological behavior of NKTL.
