CAJ | 학술논문

[目的]研究HDS基因问的同源性及其进化关系。【方法】以毛果杨的HDS基因为研究对象，利用生物信息学软件及网站对其进行碱基分布、氨基酸组成、亲疏水性、保守区以及二级结构和三级结构的预测与分析。并与其他10个物种的HDS基因序列进行多重比对与进化分析。[结果]毛果杨HDS基因的单链mRNA序列长1956bp，编码由656个氨基酸组成的蛋白质，该蛋白质相对分子质量为72937．03，其中含量最高的是Leu，为10．50％；整个多肽中疏水性氨基酸只占39．63％，有10个亲水区和6个疏水区；与其他10个物种同源性多重比对发现同源性最高达99％。[结论]毛果杨HDS基因处于稳定状态，编码的蛋白为亲水性蛋白，在进化过程中是保守的；试验中获得的保守区段序列信息为新基因的克隆奠定了基础。
[목적]연구HDS기인문적동원성급기진화관계。【방법】이모과양적HDS기인위연구대상，이용생물신식학연건급망참대기진행감기분포、안기산조성、친소수성、보수구이급이급결구화삼급결구적예측여분석。병여기타10개물충적HDS기인서렬진행다중비대여진화분석。[결과]모과양HDS기인적단련mRNA서렬장1956bp，편마유656개안기산조성적단백질，해단백질상대분자질량위72937．03，기중함량최고적시Leu，위10．50％；정개다태중소수성안기산지점39．63％，유10개친수구화6개소수구；여기타10개물충동원성다중비대발현동원성최고체99％。[결론]모과양HDS기인처우은정상태，편마적단백위친수성단백，재진화과정중시보수적；시험중획득적보수구단서렬신식위신기인적극륭전정료기출。
[Objective] The aim was to study on the homology and evolutionary rela- tionship among HAD genes. [Method] Based on HDS gene of Populus trichocarpa, bioinformatics software and website were used to predict and analyze base distribu- tion, amino acids composition, relationship with water, hyperconservative region, sec- ondary structure, and three-level structure. The results were used to compare with other HDS gene sequences of other ten species and related evolution analysis was followed. [Result] mRNA, single chain of HDS gene of Populus trichocarp＆ was 1 956 bp in length, code consisted of proteins made up of 656 amino acids, and the rela- tive molecular mass was 72 937.03 with Leu as the highest of 10.50%; in polypep- tide, hydrophobic amino acid was only of 39.63% with hydrophilc region of 10 and hydrophobic region of 6; after many comparisons with other ten species, homology was as high as 99%. [Conclusion] HDS gene of Populus trichocarpa is quite stable and albumen of code is hydrophilic protein which is quite conservative in evolution; sequence information in hyperconservative region gained in the test paved the way for cloning of novel genes.