CAJ | 학술논문

目的探讨雌二醇对肝星状细胞(HSC)的影响及其可能的作用途径.方法培养大鼠HSC,初次传代后,HSC随机分为4组,分别加入不同浓度的雌二醇,噻唑蓝(MTT)法检测雌二醇对HSC增殖的影响;逆转录-聚合酶链式反应(RT-PCR)分析其对HSC的基质金属蛋白酶因子(MMP-2)、组织金属蛋白酶抑制剂(TIMP-1)的mRNA表达的影响;免疫细胞化学分析其对HSC的α-SMA蛋白的表达的影响.结果 MTT法发现雌二醇抑制HSC的增殖,并且随浓度增加抑制作用增强,与阳性对照组比较雌二醇干预组HSC MMP-2mRNA表达增加(P<0.01),TIMP-1mRNA的表达下降(P<0.01),α-SMA蛋白表达明显下降(P<0.05).结论雌二醇具有抑制肝纤维化作用是因为:①可以抑制HSC的增殖、活化;②促进MMP-2mRNA的表达,抑制了TIMP-1mRNA的表达.
목적 탐토자이순대간성상세포(HSC)적영향급기가능적작용도경.방법 배양대서HSC,초차전대후,HSC수궤분위4조,분별가입불동농도적자이순,새서람(MTT)법검측자이순대HSC증식적영향;역전록-취합매련식반응(RT-PCR)분석기대HSC적기질금속단백매인자(MMP-2)、조직금속단백매억제제(TIMP-1)적mRNA표체적영향;면역세포화학분석기대HSC적α-SMA단백적표체적영향.결과 MTT법발현자이순억제HSC적증식,병차수농도증가억제작용증강,여양성대조조비교자이순간예조HSC MMP-2mRNA표체증가(P<0.01),TIMP-1mRNA적표체하강(P<0.01),α-SMA단백표체명현하강(P<0.05).결론 자이순구유억제간섬유화작용시인위:①가이억제HSC적증식、활화;②촉진MMP-2mRNA적표체,억제료TIMP-1mRNA적표체.
Objective To analyze the effect and the pathway of estradiol on hepatic stellate cells (HSC). Methods Cultured HSC were randomly divided into 4 groups and different concentrations of estradiol were added, then the effect of estradiol on the proliferation and the expression of MMP-1 and TIMP-1 mRNA, and α-SMA protein were detected by MTT assay, RT-PCR and Immunocytochemistry, respectively. Results The results showed that estradiol could inhibit HSCs proliferation in the dose-dependent manner. We found that estradiol up regulated the expression of MMP-2 (P<0.01) and down-regulated the expression of TIMP-1 in mRNA level (P <0.01). The expression of α-SMA protein significantly decreased as compared with the negative control (P<0. 05).Conclusion Estradiol has anti-fibrosis function. This maybe related to increase the expression of MMP-2 and decrease the expression of TIMP-1.