中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2008年
10期
983-985
,共3页
王永连%陶义鹏%王毅%王忠民%李汉臣%赵国昌%杨承汉
王永連%陶義鵬%王毅%王忠民%李漢臣%趙國昌%楊承漢
왕영련%도의붕%왕의%왕충민%리한신%조국창%양승한
食管癌%血管内皮生长因子%PENT基因%免疫组织化学
食管癌%血管內皮生長因子%PENT基因%免疫組織化學
식관암%혈관내피생장인자%PENT기인%면역조직화학
Esophageal carcinoma%Vascular endothelial growth factors%PTEN genes%Immunohisto chemitry
目的 通过对PTEN和血管内皮生长因子(VEGF)在食管癌中表达的研究,结合临床病理特征,探讨PTEN和VEGF的临床意义及相关性.方法 应用免疫组织化学S-P法检测80例食管癌组织及20例正常食管黏膜中PTEN和VEGF的表达.结果 食管癌组织中PTEN蛋白表达率为38.75%(31/80),显著低于正常食管黏膜的表达(100%,20/20)(P<0.01),PTEN蛋白低表达与组织分化程度密切相关(r=0.57,P<0.05),与淋巴结转移密切相关(r=0.61,P<0.01).VEGF在食管癌中的表达率为70.13%(57/80).显著高于正常食管黏膜的表达(15.0%,3/20)(P<0.01),VEGF的表达与癌组织浸润深度密切相关(r=0.49,P<0.05),与淋巴结转移也密切相关(r=0.55,P<0.05),与组织分化程度相关(r=0.48,P<0.05).PTEN在食管癌中的表达与VEGF呈负相关(r=-0.327,P<0.01).结论 联合检测PTEN、VEGF 有助于提高食管癌侵袭转移能力的评估,对食管癌的预后判断具有重要的临床意义.
目的 通過對PTEN和血管內皮生長因子(VEGF)在食管癌中錶達的研究,結閤臨床病理特徵,探討PTEN和VEGF的臨床意義及相關性.方法 應用免疫組織化學S-P法檢測80例食管癌組織及20例正常食管黏膜中PTEN和VEGF的錶達.結果 食管癌組織中PTEN蛋白錶達率為38.75%(31/80),顯著低于正常食管黏膜的錶達(100%,20/20)(P<0.01),PTEN蛋白低錶達與組織分化程度密切相關(r=0.57,P<0.05),與淋巴結轉移密切相關(r=0.61,P<0.01).VEGF在食管癌中的錶達率為70.13%(57/80).顯著高于正常食管黏膜的錶達(15.0%,3/20)(P<0.01),VEGF的錶達與癌組織浸潤深度密切相關(r=0.49,P<0.05),與淋巴結轉移也密切相關(r=0.55,P<0.05),與組織分化程度相關(r=0.48,P<0.05).PTEN在食管癌中的錶達與VEGF呈負相關(r=-0.327,P<0.01).結論 聯閤檢測PTEN、VEGF 有助于提高食管癌侵襲轉移能力的評估,對食管癌的預後判斷具有重要的臨床意義.
목적 통과대PTEN화혈관내피생장인자(VEGF)재식관암중표체적연구,결합림상병리특정,탐토PTEN화VEGF적림상의의급상관성.방법 응용면역조직화학S-P법검측80례식관암조직급20례정상식관점막중PTEN화VEGF적표체.결과 식관암조직중PTEN단백표체솔위38.75%(31/80),현저저우정상식관점막적표체(100%,20/20)(P<0.01),PTEN단백저표체여조직분화정도밀절상관(r=0.57,P<0.05),여림파결전이밀절상관(r=0.61,P<0.01).VEGF재식관암중적표체솔위70.13%(57/80).현저고우정상식관점막적표체(15.0%,3/20)(P<0.01),VEGF적표체여암조직침윤심도밀절상관(r=0.49,P<0.05),여림파결전이야밀절상관(r=0.55,P<0.05),여조직분화정도상관(r=0.48,P<0.05).PTEN재식관암중적표체여VEGF정부상관(r=-0.327,P<0.01).결론 연합검측PTEN、VEGF 유조우제고식관암침습전이능력적평고,대식관암적예후판단구유중요적림상의의.
Objective To investigate the expression of PTEN and vascular endothelial growth factor (VEGF) in esophageal carcinoma and the relationship between their expression.Methods The expression of PTEN and VEGF were detected using immunohistochemical S-P method.Results Among 80 cases of esophageal carcino- ma,31 showed positive staining of PTEN (38.75%),while all 20 case of normal mucosa showed positiva staimng of PTEN.The expression level of PTEN in highly differentiated squamous carcinoma was higher than that low differ- entiated squamous carcinoma.Also,the expression of PTEN was significantly correlated with lymph node metastasis (r=0.61,P<0.01)and differentiation(r=0.57.P<0.05).In 80 cases of esophageal carcinoma,57(70.13%) were of positive staining of VEGF,while in 20 of normal esophageal mucosa,only 3 showed positive staining of VEGF.The expression of VEGF was markedly correlated with infiltrative deepness(r=0.49,P<0.05)and lymph node metastasis(r=0.55,P<0.05)and differentiation(r=0.48,P<0.05).Conclusion Combined detection of PTEN and VEGF maybe helpful to evaluate prognosis and infiltrative capability of esophageal carcinoma,with sig- nificant importance to the prediction of the prognosis of esophageal carcinoam.
