CAJ | 학술논문

目的研究ER、PR、nm23及PTEN基因蛋白在乳腺癌中的表达及临床病理意义.方法采用免疫组织化学S-P法.检测石腊包埋42例乳腺癌组织和12例乳腺小叶增生中PTEN蛋白、nm23蛋白、ER和PR的表达水平.结果 PTEN在乳腺癌高表达比乳腺小叶增生低(P＜0.05).PTEN基因表达与肿瘤大小、临床分期、组织分级和肿瘤复发无相关性,小于45岁患者PTEN表达比大于45岁偏低(P＜0.05);PTEN高表达与腋淋巴结转移情况有显著负相关性(P＜0.01);与ER受体呈正相关性.PTEN基因高表达与远处转移有负相关性(P＜0.05);与5年生存率呈正相关性(P＜0.01).乳腺恶性肿瘤组织中nm23高表达率与良性组织比较差异无显著性(P＞0.05).nm23基因表达与肿瘤大小、临床分期、组织分级、肿瘤复发及年龄无相关性,nm23高表达与腋淋巴结转移情况有显著正相关性(P＜0.001),nm23高表达与患者远处转移有显著负相关性(P＜0.001).与患者5年生存率有正相关性(P＜0.05).结论 nm23与PTEN表达异常与乳腺癌发生发展密切相关,联合检测ER、PR、nm23及PTEN基因的表达可作为一种预测乳腺肿瘤预后和恶性程度的指标.
목적 연구ER、PR、nm23급PTEN기인단백재유선암중적표체급림상병리의의.방법 채용면역조직화학S-P법.검측석석포매42례유선암조직화12례유선소협증생중PTEN단백、nm23단백、ER화PR적표체수평.결과 PTEN재유선암고표체비유선소협증생저(P＜0.05).PTEN기인표체여종류대소、림상분기、조직분급화종류복발무상관성,소우45세환자PTEN표체비대우45세편저(P＜0.05);PTEN고표체여액림파결전이정황유현저부상관성(P＜0.01);여ER수체정정상관성.PTEN기인고표체여원처전이유부상관성(P＜0.05);여5년생존솔정정상관성(P＜0.01).유선악성종류조직중nm23고표체솔여량성조직비교차이무현저성(P＞0.05).nm23기인표체여종류대소、림상분기、조직분급、종류복발급년령무상관성,nm23고표체여액림파결전이정황유현저정상관성(P＜0.001),nm23고표체여환자원처전이유현저부상관성(P＜0.001).여환자5년생존솔유정상관성(P＜0.05).결론 nm23여PTEN표체이상여유선암발생발전밀절상관,연합검측ER、PR、nm23급PTEN기인적표체가작위일충예측유선종류예후화악성정도적지표.
Objective To explore the expressions of PTEN gene, nm23 gene, progesterone receptor, and estrogen receptor in breast cancer and their clinical, values. Methods The expression levels of PTEN, nm23, progesterone receptor, and estrogen receptor were detected by immunohistochemistry(S-P method)in 42 samples of paraffin-embedded human breast cancer tissues and 12 samples of hyperplasia of mammary gland tissues. Results The high expression rate of PTEN gene was lower in breast cancer than in hyperplasia of mammary glands(P＜ 0.05). The expression of PTEN gene was not related with tumor size, histological grade, clinical stage and postoperative recurrence; but the high expression rate was higher in elder patients(＞ 45 years)than in young patients(＜ 45 years). The high expression of PTEN was significantly inversely correlated with axillary lymph node metastasis(P＜ 0.01), and had positive relationship with estrogen receptor(P＜ 0.05). The high expression of PTEN was negatively related with distant metastasis(P＜ 0.05), and had positive relationship with 5-year survival rate(P＜ 0.01). The high expression rate of nm23 gene did not differ significantly between breast cancer and hyperplasia of mammary glands(P＞ 0.05). The expression of nm23 gene was not related with tumor size, histological grade, clinical stage, postoperative recurrence, and age. The high expression of nm23 was significantly positively correlated with axillary lymph node metastasis(P＜ 0.01), but was negatively correlated with distant metastasis(P＜ 0.05), and had positive relationship with 5-year survival rate(P ＜ 0.01). Conclusions Abnormal expressions of PTEN gene and nm23 gene are closely associated with the tumorigenesis and development of primary breast cancer. Combined detection of expressions of PTEN, nm23, progesterone receptor and estrogen receptor can be used as a predictor for biological behaviors and prognosis of breast cancer.