CAJ | 학술논문

目的建立痤疮搽剂中氯霉素、水杨酸的含量测定方法.方法以UltimateTM XB-C18为分析柱,乙腈-0.01 mol/L庚烷磺酸钠缓冲溶液(取磷酸二氢钾6.8 g,用0.01 mol/L庚烷磺酸钠溶液溶解并稀释至1000ml,再加三乙胺5ml,混匀,用磷酸调节pH值至2.5)(25:75)为流动相;流速:1.0ml·min-1;检测波长:278 nm;进样量10 μl.结果氯霉素对照品在29.49～196.60μg/ml范围内,浓度与峰面积呈良好的线性关系,r=0.999 9,平均回收率为99.1%;水杨酸对照品在31.41～209.40μg/ml,范围内具有良好的线性关系,r=0.9999,平均回收率为99.5%.结论本测定方法简便、准确,可用于痤疮搽剂的质量控制.
목적 건립좌창차제중록매소、수양산적함량측정방법.방법 이UltimateTM XB-C18위분석주,을정-0.01 mol/L경완광산납완충용액(취린산이경갑6.8 g,용0.01 mol/L경완광산납용액용해병희석지1000ml,재가삼을알5ml,혼균,용린산조절pH치지2.5)(25:75)위류동상;류속:1.0ml·min-1;검측파장:278 nm;진양량10 μl.결과 록매소대조품재29.49～196.60μg/ml범위내,농도여봉면적정량호적선성관계,r=0.999 9,평균회수솔위99.1%;수양산대조품재31.41～209.40μg/ml,범위내구유량호적선성관계,r=0.9999,평균회수솔위99.5%.결론 본측정방법간편、준학,가용우좌창차제적질량공제.
Objective To establish a method for detecting the contents of chloramphenicol and salicylic acid in Acne liniment by HPLC. Methods HPLC was conducted on a UltimateTM XB-C18with acetonitrile-0.01 mol/L sodium heptanesulfonate solution (dissove 6.8 g,potassium phosphate monobasic and mixing well,adjusting to pH 2.5 with phosphoric acid) (25:75) as mobile phase,detected at 278 nm;with a flow rate of 1.0 ml·min-1. Results The calibration curve of chloramphenicol was linear with 29.49 ～ 196.60 μg/ml (r = 0.9999). The average recovery of chloramphenicol was 99.1％. The calibration curve = 0.9999). The average recovery of chloramphenicol was 99.1％. The calibration curve of salicylic acid was linear with 31.41 ～ 209.40 μg/ml (r = 0.999 9). The average recovery of Salicylic acid was 99.5％.Conclusions This detection method is simple and accurate and can be used for the quality control of Acne liniment.