CAJ | 학술논문

目的通过对SFRP2基因在结直肠癌和癌旁组织中的超甲基化水平的测定,分析其与结直肠癌临床病理特征的关系.探讨SFRP2基因与结直肠癌浸润、转移等的关系.方法用实时定量聚合酶链反应技术检测分析30例结直肠癌患者癌组织、癌旁组织中SFRP2基因超甲基化水平,并统计学分析其与临床病理特征的关系.结果在癌组织和癌旁组织中均有SFRP2基因超甲基化发生；TNM分期中结直肠癌Ⅲ、Ⅳ期(7.24±1.13)组织中甲基化水平明显高于结直肠癌Ⅰ、Ⅱ期(5.92 ±0.97)组织中甲基化水平(P＜0.05)；在低分化结直肠癌组织中(7.31±1.11)甲基化水平明显高于中高分化结直肠癌癌组织(6.23±1.03),差异有统计学意义(P＜0.05)；与性别、年龄、肿块位置及大小差异无统计学意义(P＞0.05).结论 SFRP2基因超甲基化对结直肠癌恶性程度、浸润、转移和预后判定具有重要的意义.
목적 통과대SFRP2기인재결직장암화암방조직중적초갑기화수평적측정,분석기여결직장암림상병리특정적관계.탐토SFRP2기인여결직장암침윤、전이등적관계.방법 용실시정량취합매련반응기술검측분석30례결직장암환자암조직、암방조직중SFRP2기인초갑기화수평,병통계학분석기여림상병리특정적관계.결과 재암조직화암방조직중균유SFRP2기인초갑기화발생；TNM분기중결직장암Ⅲ、Ⅳ기(7.24±1.13)조직중갑기화수평명현고우결직장암Ⅰ、Ⅱ기(5.92 ±0.97)조직중갑기화수평(P＜0.05)；재저분화결직장암조직중(7.31±1.11)갑기화수평명현고우중고분화결직장암암조직(6.23±1.03),차이유통계학의의(P＜0.05)；여성별、년령、종괴위치급대소차이무통계학의의(P＞0.05).결론 SFRP2기인초갑기화대결직장암악성정도、침윤、전이화예후판정구유중요적의의.
Objective To explore the differential level of hypermethylated SFRP2 gene in colorectal cancers andadjacent nontumorous tissues,to analyze the relation of SFRP2 gene promoter hypermethylation status and its clinicopathologic significance in colorectal carcinoma,and to study the relationship between the level of hypermethylated SFRP2 and the invasion and metastasis of colorectal carcinoma.Methods Real-time quantitative PCR technique was performed to analyze the level of hypermethylated SFRP2 gene promoter in colorectal cancers and adjacent nontumorous tissues taken from 30 colorectal cancer patients.The relation of the level of hypermethylated SFRP2 gene promoter and its clinicopathologic features of colorectal cancers was analyzed.Results SFRP2 gene promoter hypermethylation occurred in both the tumor tissues and the adjacent tissues.The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with TNM Ⅲ and Ⅳ (7.24 ± 1.13)than in patients with TNM Ⅰ and Ⅱ ( 5.92 ± 0.97 ) ( P ＜ 0.05 ).The level of SFRP2 gene promoter hypermethylation was significantly higher in the patients with lower differentiation(7.31 ± 1.11 ) than in patients with higher differentiation (6.23 ± 1.03) ( P ＜ 0.05 ).No significant association was found between the level of SFRP2 gene promoter hypermethylation and the status of gender,age.tumor location and tumor size ( P ＞ 0.05 ).Conclusions SFRP2 gene hypermethylation in tissues may be a useful objective parameter for the malignant level,invasion,metastasis,recurrence and prognosis of the colorectal cancer.