中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
11期
845-850
,共6页
于丹%金春顺%赵胤%许承弼
于丹%金春順%趙胤%許承弼
우단%금춘순%조윤%허승필
肿瘤细胞,培养的%肿瘤干细胞%喉肿瘤%癌,鳞状细胞
腫瘤細胞,培養的%腫瘤榦細胞%喉腫瘤%癌,鱗狀細胞
종류세포,배양적%종류간세포%후종류%암,린상세포
Tumor cells,cultured%Tumor stem cells%Laryngeal neoplasms%Carcinoma,squamous cell
目的 初步研究透明质酸盐受体(hyalurate receptor)CD+44喉癌细胞的干细胞生物学特性.方法 原代培养喉痛细胞,利用亲和板结合分离法,将CD+44和CD+44肿瘤细胞分选出来,继续常规培养,比较CD+44与CD+44肿瘤细胞在功能状态、细胞周期、分化状态、克隆形成能力及形态等方面的差异.结果 CD+44细胞在喉癌细胞中所占的百分率为49.8%～53.5%,中位数为51.3%,亲和板结合法分选出CD+44细胞,继续常规培养后恢复原始比例.CD+44细胞中RNA含量低,细胞处于GO和G1期的比例高于CD+44细胞.CD+44细胞中正常鳞状上皮干细胞角蛋白(cytokeratin 14,CK14)表达强阳性或阳性,而分化的上皮细胞标记物外皮蛋白(involucrin)表达弱阴性或阴性.倒置显微镜下观察,CD+44细胞为多角形,突起多;而CD+44细胞为梭形,突起较少.对CD+44细胞进行有限稀释单细胞克隆培养,产生的子代克隆集落出现了异质性细胞,而CD-44细胞未能产生异质性子代细胞.与CD-44细胞相比,CD+44细胞表现出较高的克隆形成能力说明其具有较强的增殖能力.结论 CD+44喉癌细胞具有干细胞的某些特性,肿瘤干细胞可能存在于CD+44细胞内.
目的 初步研究透明質痠鹽受體(hyalurate receptor)CD+44喉癌細胞的榦細胞生物學特性.方法 原代培養喉痛細胞,利用親和闆結閤分離法,將CD+44和CD+44腫瘤細胞分選齣來,繼續常規培養,比較CD+44與CD+44腫瘤細胞在功能狀態、細胞週期、分化狀態、剋隆形成能力及形態等方麵的差異.結果 CD+44細胞在喉癌細胞中所佔的百分率為49.8%～53.5%,中位數為51.3%,親和闆結閤法分選齣CD+44細胞,繼續常規培養後恢複原始比例.CD+44細胞中RNA含量低,細胞處于GO和G1期的比例高于CD+44細胞.CD+44細胞中正常鱗狀上皮榦細胞角蛋白(cytokeratin 14,CK14)錶達彊暘性或暘性,而分化的上皮細胞標記物外皮蛋白(involucrin)錶達弱陰性或陰性.倒置顯微鏡下觀察,CD+44細胞為多角形,突起多;而CD+44細胞為梭形,突起較少.對CD+44細胞進行有限稀釋單細胞剋隆培養,產生的子代剋隆集落齣現瞭異質性細胞,而CD-44細胞未能產生異質性子代細胞.與CD-44細胞相比,CD+44細胞錶現齣較高的剋隆形成能力說明其具有較彊的增殖能力.結論 CD+44喉癌細胞具有榦細胞的某些特性,腫瘤榦細胞可能存在于CD+44細胞內.
목적 초보연구투명질산염수체(hyalurate receptor)CD+44후암세포적간세포생물학특성.방법 원대배양후통세포,이용친화판결합분리법,장CD+44화CD+44종류세포분선출래,계속상규배양,비교CD+44여CD+44종류세포재공능상태、세포주기、분화상태、극륭형성능력급형태등방면적차이.결과 CD+44세포재후암세포중소점적백분솔위49.8%～53.5%,중위수위51.3%,친화판결합법분선출CD+44세포,계속상규배양후회복원시비례.CD+44세포중RNA함량저,세포처우GO화G1기적비례고우CD+44세포.CD+44세포중정상린상상피간세포각단백(cytokeratin 14,CK14)표체강양성혹양성,이분화적상피세포표기물외피단백(involucrin)표체약음성혹음성.도치현미경하관찰,CD+44세포위다각형,돌기다;이CD+44세포위사형,돌기교소.대CD+44세포진행유한희석단세포극륭배양,산생적자대극륭집락출현료이질성세포,이CD-44세포미능산생이질성자대세포.여CD-44세포상비,CD+44세포표현출교고적극륭형성능력설명기구유교강적증식능력.결론 CD+44후암세포구유간세포적모사특성,종류간세포가능존재우CD+44세포내.
Objective To study the biological characteristics of CD+44 stem cells in human laryngeal carcinoma.Methods Tumor samples were obtained from 5 patients, and then the human laryngeal carcinoma cells were cultured in vitro by primary tissue culture technique.Taking CD44 molecule as a marker to isolate CD+44 subpopulation cells from laryngeal carcinoma cells for further study.CD+44 and CD-44 cells were cultured and observed fex their development.CD+44 and CD-44 cells were compared in their functional status ( mRNA), cell cycles (GO/G1), their degree of differentiation (CK14 and Involucrin expression) and their morphologic character of the clone.Results The percentages of CD+44 cells were about49.8% -53.5% and the median was 51.3%.After culturing CD+44 cells isolated from laryngeal carcinoma could proliferate and the percentage of CD+44 remained the same.CD+44 tumor cells contained much less RNA, more GO/GI cells, expressed more CK14 protein and less lnvolucrin protein (less differentiated state).CD+44 cells were muhangular in shape with protuberances; CD-44celis showed a sharp and spindle feature.In comparison with CD-44 cells, CD+44 cells could create heterogeneous offspring by single cell culture of limiting dilution.By observing clone forming rate after single cell planting, it was found that the CD+44 cells had stronger proliferation ability.Conclusions CD+44 cells possess some characteristics of stem cells, laryngeal carcinoma stem cells maybe exist in CD+44 cells.
