CAJ | 학술논문

目的探讨真核翻译起始因子4E(eukaryotic translation initiation factor 4E,eIF4E)在病理性瘢痕组织中的表达及其在病理性瘢痕形成中的作用及机制.方法 (1)应用免疫组织化学SP法检测20例正常皮肤、20例成熟瘢痕、14例增生性瘢痕和25例瘢痕疙瘩组织中eIF4E蛋白的表达.(2)应用逆转录-聚合酶链式反应(RT-PCR)测定eIF4E mRNA在8例正常皮肤、8例成熟瘢痕、7例增生性瘢痕和8例瘢痕疙瘩组织中的半定量值.结果 (1)病理性瘢痕组织中eIF4E蛋白表达增高,与正常皮肤、成熟瘢痕对照组比较,差异有统计学意义(P＜0.05).(2)与正常对照组0.99±0.28比较,eIF4E mRNA在病理性瘢痕组织1.73±0.31中的表达显著增高,差异有统计学意义(P＜0.05).结论 eIF4E在病理性瘢痕组织中表达增高,eIF4E与病理性瘢痕的形成密切相关,可能对病理性瘢痕的形成起着重要作用.
목적 탐토진핵번역기시인자4E(eukaryotic translation initiation factor 4E,eIF4E)재병이성반흔조직중적표체급기재병이성반흔형성중적작용급궤제.방법 (1)응용면역조직화학SP법검측20례정상피부、20례성숙반흔、14례증생성반흔화25례반흔흘탑조직중eIF4E단백적표체.(2)응용역전록-취합매련식반응(RT-PCR)측정eIF4E mRNA재8례정상피부、8례성숙반흔、7례증생성반흔화8례반흔흘탑조직중적반정량치.결과 (1)병이성반흔조직중eIF4E단백표체증고,여정상피부、성숙반흔대조조비교,차이유통계학의의(P＜0.05).(2)여정상대조조0.99±0.28비교,eIF4E mRNA재병이성반흔조직1.73±0.31중적표체현저증고,차이유통계학의의(P＜0.05).결론 eIF4E재병이성반흔조직중표체증고,eIF4E여병이성반흔적형성밀절상관,가능대병이성반흔적형성기착중요작용.
Objective To study the expression of eukaryotic translation initiation factor 4E(eIF4E)in the pathological scars and its probable role in the pathogenesis of pathological scars.Methods Immunohistochemiscal technique was performed to detect the expression and distribution of eIF4E protein in hypertrophic scars(14 cases),keloids(25 cases),mature scars(20 cases)and normal skins(20 cases).Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the eIF4E mRNA level in hypertrophic scars(7 cases),keloids(8 cases),mature scars(8 cases)and normal skins(8 cases).Results Thepositive rate of eIF4E protein expression was remarkably significant difference between normal scars and pathological scars(P＜0.05).The level of eIF4E mRNA in pathological scars 1.73±0.31was higher than that in control group 0.99±0.28.There was significant difference between two groups (P＜O.05).Conclusions The expression of eIF4E is increased in pathological scar.eIF4 E expression is closely associated with the development of pathological scar.Therefore,eIF4E overexpression may play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar.