中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2009年
3期
164-167
,共4页
孙蕾民%刘玮丽%商燕%姒健敏
孫蕾民%劉瑋麗%商燕%姒健敏
손뢰민%류위려%상연%사건민
慢性萎缩性胃炎%胃肿瘤%热休克蛋白
慢性萎縮性胃炎%胃腫瘤%熱休剋蛋白
만성위축성위염%위종류%열휴극단백
Gastritis%atrophic%Stomach neoplasms%Heat shock proteins
目的 探讨热休克蛋白(HSP)90和HSP70在慢性萎缩性胃炎及胃癌中的表达及意义.方法 收集2005年4月至2006年4月慢性浅表性胃炎患者35例、慢性萎缩性胃炎患者66例(轻度21例、中度30例、重度15例)和胃癌患者40例.中位年龄分别为47.8、56.1和59.4岁.幽门螺杆菌(Hp)阳性在慢性浅表性胃炎和萎缩性胃炎者中分别为12例和28例.HSP70和HSP90的定性检测采用免疫组化法,基因和蛋白检测分别采用Western印迹和实时荧光聚合酶链反应法.结果 HSP70基因表达在萎缩性胃炎(1.41±0.80)中高于浅表性胃炎(1.31±0.80)和胃癌(1.18±0.70),差异有统计学意义(P＜0.05);且在轻、中度萎缩者中呈低表达.分别为1.32±0.70和1.34±0.60,而在重度萎缩者中明显升高(2.20±0.80,P＜0.05),HSP90的基因表达从浅表性胃炎(1.27±0.60)、萎缩性胃炎(1.53±O.80)至胃癌(1.84±0.70)呈逐步升高趋势;且在轻度(1.33±0.60)、中度(1.47±0.90)、重度(2.75±0.70)萎缩性胃炎中亦呈逐步升高趋势(P＜0.05).HSP90表达在Hp阳性(1.63±0.80)萎缩性胃炎患者中高于阴性患者(1.35±0.50).两者间差异有统计学意义(P＜0.05).结论 HSP70和HSP90同步升高预示慢性萎缩性胃炎的萎缩程度加重,而当HSP70下降而HSP90上升时提示胃癌发生可能.
目的 探討熱休剋蛋白(HSP)90和HSP70在慢性萎縮性胃炎及胃癌中的錶達及意義.方法 收集2005年4月至2006年4月慢性淺錶性胃炎患者35例、慢性萎縮性胃炎患者66例(輕度21例、中度30例、重度15例)和胃癌患者40例.中位年齡分彆為47.8、56.1和59.4歲.幽門螺桿菌(Hp)暘性在慢性淺錶性胃炎和萎縮性胃炎者中分彆為12例和28例.HSP70和HSP90的定性檢測採用免疫組化法,基因和蛋白檢測分彆採用Western印跡和實時熒光聚閤酶鏈反應法.結果 HSP70基因錶達在萎縮性胃炎(1.41±0.80)中高于淺錶性胃炎(1.31±0.80)和胃癌(1.18±0.70),差異有統計學意義(P＜0.05);且在輕、中度萎縮者中呈低錶達.分彆為1.32±0.70和1.34±0.60,而在重度萎縮者中明顯升高(2.20±0.80,P＜0.05),HSP90的基因錶達從淺錶性胃炎(1.27±0.60)、萎縮性胃炎(1.53±O.80)至胃癌(1.84±0.70)呈逐步升高趨勢;且在輕度(1.33±0.60)、中度(1.47±0.90)、重度(2.75±0.70)萎縮性胃炎中亦呈逐步升高趨勢(P＜0.05).HSP90錶達在Hp暘性(1.63±0.80)萎縮性胃炎患者中高于陰性患者(1.35±0.50).兩者間差異有統計學意義(P＜0.05).結論 HSP70和HSP90同步升高預示慢性萎縮性胃炎的萎縮程度加重,而噹HSP70下降而HSP90上升時提示胃癌髮生可能.
목적 탐토열휴극단백(HSP)90화HSP70재만성위축성위염급위암중적표체급의의.방법 수집2005년4월지2006년4월만성천표성위염환자35례、만성위축성위염환자66례(경도21례、중도30례、중도15례)화위암환자40례.중위년령분별위47.8、56.1화59.4세.유문라간균(Hp)양성재만성천표성위염화위축성위염자중분별위12례화28례.HSP70화HSP90적정성검측채용면역조화법,기인화단백검측분별채용Western인적화실시형광취합매련반응법.결과 HSP70기인표체재위축성위염(1.41±0.80)중고우천표성위염(1.31±0.80)화위암(1.18±0.70),차이유통계학의의(P＜0.05);차재경、중도위축자중정저표체.분별위1.32±0.70화1.34±0.60,이재중도위축자중명현승고(2.20±0.80,P＜0.05),HSP90적기인표체종천표성위염(1.27±0.60)、위축성위염(1.53±O.80)지위암(1.84±0.70)정축보승고추세;차재경도(1.33±0.60)、중도(1.47±0.90)、중도(2.75±0.70)위축성위염중역정축보승고추세(P＜0.05).HSP90표체재Hp양성(1.63±0.80)위축성위염환자중고우음성환자(1.35±0.50).량자간차이유통계학의의(P＜0.05).결론 HSP70화HSP90동보승고예시만성위축성위염적위축정도가중,이당HSP70하강이HSP90상승시제시위암발생가능.
Objective To investigate the expression of heat shock protein(HSP)70 and HSP90 in patients with atrophic gastritis(AG)or gastric cancer(GC)and its significance.Methods One hundred and forty-one patients including 35 with superficial gastritis(CSG),66 with AG(miner in 21,medium in 30 and severe in 15)and 40 with GC were enrolled with mean age of 47.8,56.1 or 59.4 years,respectively.H.pylori positive patients were 12 with CSG and 28 with AG.The quantity analysis of HSP90 and HSP70 were detected by immunohistochemistry,whereas their mRNA and protein expressions were measured by Western bolt and real-time PCR,respectively.Results The mRNA expression of HSP70 was significantly higher in CSG(1.31±0.80)and AG(1.41±0.80)than that in GC(1.18±0.70,P＜0.05),but was significantly lower in miner(1.32±0.70)and medium(1.34±0.60)AG than that in severe AG(2.20±0.80,P＜0.05).The mRNA expression of HSP90 was kept on increasing in order of CSG(1.27±0.60),AG(1.53±0.80)and GC(1.84±0.70); or in order of miner(1.33±0.60),medium(1.47±0.90)and severe(2.75±0.70)(P＜0.05).The patients infected with H.pylori had higher expression of HSP90 compared with those without H. pylori infection (P＜0.05). Conclusion The synchronous increase of HSP90 and HSP70 indicates the aggravation of AG. When HSP90 is up-regulated and HSP70 is down-regulatied, it may predict the occurrence of cancer.
