中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2012年
3期
170-176
,共7页
褚秀丽%赵玉武%米亚静%沈洁%王喜云%刘建芝%金卫林
褚秀麗%趙玉武%米亞靜%瀋潔%王喜雲%劉建芝%金衛林
저수려%조옥무%미아정%침길%왕희운%류건지%금위림
低血糖%脑损伤%海马
低血糖%腦損傷%海馬
저혈당%뇌손상%해마
Hypoglycemia%Brain injury%Hippocampus
目的 探讨胰岛素诱导大鼠低血糖后,血糖升高水平对大鼠低血糖性脑损害的影响.方法 30只Wistar4月龄雄性大鼠,体重(300±50)g,用简单随机抽样方法 分为实验组(20只)、正常血糖对照组(A组,5只)和空白对照组(B组,5只).实验组根据血糖再灌注水平分为1＜血糖≤3 mmol/L组(C组)、3＜血糖≤6 mmol/L组(D组)、6＜血糖≤9 mmol/L组(E组)、血糖＞9 mmol/L组(F组),每组5只.采用TUNEL染色观察大鼠海马神经元凋亡,Fluoro-Jade B(FJB)染色观察神经元轴突和胞体的退变.染色组间计量资料采用单因素方差分析.结果 (1) TUNEL染色:与A组及B组相比(海马CA1区:3.2±1.9、2.8±0.8；海马DG区:4.1±2.4、3.4±1.2),C组、D组、E组、F组海马凋亡细胞数(海马CA1区:40.2±3.1、38.7±2.4、36.8±2.6和76.4±6.3；海马DG区:62.4±4.2、59.8±3.7、68.1±2.8和125.4±5.8)凋亡细胞数增多,差异有统计学意义(海马CA1区:F=13.52,P＜0.05；海马DG区:F=14.29,P＜0.05)；F组(海马CA1区:76.4±6.3；海马DG区:125.4±5.8)大鼠海马凋亡神经元数目比C组、D组、E组(分别为海马CA1区:40.2±3.1、38.7±2.4、36.8±2.6；海马DG区:62.4±4.2、59.8±3.7、68.1±2.8)显著增多,差异有统计学意义(海马CA1区:F=5.08,P＜0.05；海马DG区:F=6.52,P＜0.05)；(2) FJB染色:与A组及B组相比,C组、D组、E组、F组海马退变神经元数目显著增多,差异有统计学意义(海马CA1区:F=18.49,P＜0.05；海马DG区:F=11.37,P＜0.05)；F组大鼠海马轴突退变神经元数目比C组、D组、E组显著增多,差异有统计学意义(海马CA1区:F =7.83,P＜0.05；海马DG区:F=14.29,P＜0.05).结论 在同一低血糖水平且持续时间相同的情况下,大鼠脑损害程度与低血糖后血糖升高水平有关:血糖升高水平过高,脑损害明显.
目的 探討胰島素誘導大鼠低血糖後,血糖升高水平對大鼠低血糖性腦損害的影響.方法 30隻Wistar4月齡雄性大鼠,體重(300±50)g,用簡單隨機抽樣方法 分為實驗組(20隻)、正常血糖對照組(A組,5隻)和空白對照組(B組,5隻).實驗組根據血糖再灌註水平分為1＜血糖≤3 mmol/L組(C組)、3＜血糖≤6 mmol/L組(D組)、6＜血糖≤9 mmol/L組(E組)、血糖＞9 mmol/L組(F組),每組5隻.採用TUNEL染色觀察大鼠海馬神經元凋亡,Fluoro-Jade B(FJB)染色觀察神經元軸突和胞體的退變.染色組間計量資料採用單因素方差分析.結果 (1) TUNEL染色:與A組及B組相比(海馬CA1區:3.2±1.9、2.8±0.8；海馬DG區:4.1±2.4、3.4±1.2),C組、D組、E組、F組海馬凋亡細胞數(海馬CA1區:40.2±3.1、38.7±2.4、36.8±2.6和76.4±6.3；海馬DG區:62.4±4.2、59.8±3.7、68.1±2.8和125.4±5.8)凋亡細胞數增多,差異有統計學意義(海馬CA1區:F=13.52,P＜0.05；海馬DG區:F=14.29,P＜0.05)；F組(海馬CA1區:76.4±6.3；海馬DG區:125.4±5.8)大鼠海馬凋亡神經元數目比C組、D組、E組(分彆為海馬CA1區:40.2±3.1、38.7±2.4、36.8±2.6；海馬DG區:62.4±4.2、59.8±3.7、68.1±2.8)顯著增多,差異有統計學意義(海馬CA1區:F=5.08,P＜0.05；海馬DG區:F=6.52,P＜0.05)；(2) FJB染色:與A組及B組相比,C組、D組、E組、F組海馬退變神經元數目顯著增多,差異有統計學意義(海馬CA1區:F=18.49,P＜0.05；海馬DG區:F=11.37,P＜0.05)；F組大鼠海馬軸突退變神經元數目比C組、D組、E組顯著增多,差異有統計學意義(海馬CA1區:F =7.83,P＜0.05；海馬DG區:F=14.29,P＜0.05).結論 在同一低血糖水平且持續時間相同的情況下,大鼠腦損害程度與低血糖後血糖升高水平有關:血糖升高水平過高,腦損害明顯.
목적 탐토이도소유도대서저혈당후,혈당승고수평대대서저혈당성뇌손해적영향.방법 30지Wistar4월령웅성대서,체중(300±50)g,용간단수궤추양방법 분위실험조(20지)、정상혈당대조조(A조,5지)화공백대조조(B조,5지).실험조근거혈당재관주수평분위1＜혈당≤3 mmol/L조(C조)、3＜혈당≤6 mmol/L조(D조)、6＜혈당≤9 mmol/L조(E조)、혈당＞9 mmol/L조(F조),매조5지.채용TUNEL염색관찰대서해마신경원조망,Fluoro-Jade B(FJB)염색관찰신경원축돌화포체적퇴변.염색조간계량자료채용단인소방차분석.결과 (1) TUNEL염색:여A조급B조상비(해마CA1구:3.2±1.9、2.8±0.8；해마DG구:4.1±2.4、3.4±1.2),C조、D조、E조、F조해마조망세포수(해마CA1구:40.2±3.1、38.7±2.4、36.8±2.6화76.4±6.3；해마DG구:62.4±4.2、59.8±3.7、68.1±2.8화125.4±5.8)조망세포수증다,차이유통계학의의(해마CA1구:F=13.52,P＜0.05；해마DG구:F=14.29,P＜0.05)；F조(해마CA1구:76.4±6.3；해마DG구:125.4±5.8)대서해마조망신경원수목비C조、D조、E조(분별위해마CA1구:40.2±3.1、38.7±2.4、36.8±2.6；해마DG구:62.4±4.2、59.8±3.7、68.1±2.8)현저증다,차이유통계학의의(해마CA1구:F=5.08,P＜0.05；해마DG구:F=6.52,P＜0.05)；(2) FJB염색:여A조급B조상비,C조、D조、E조、F조해마퇴변신경원수목현저증다,차이유통계학의의(해마CA1구:F=18.49,P＜0.05；해마DG구:F=11.37,P＜0.05)；F조대서해마축돌퇴변신경원수목비C조、D조、E조현저증다,차이유통계학의의(해마CA1구:F =7.83,P＜0.05；해마DG구:F=14.29,P＜0.05).결론 재동일저혈당수평차지속시간상동적정황하,대서뇌손해정도여저혈당후혈당승고수평유관:혈당승고수평과고,뇌손해명현.
Objective To explore the influence of the rising blood glucose level on brain injury in rats after insulin-induced hypoglycemia.Methods A total of 30 Wistar rats ( weight:( 300 ± 50) g,age:4 months) were simpling randomly divided into three equal groups:experimental group(20 rats),both vehicle control group (group A,5 rats)and normal control group (group B,5 rats).According to the blood glucose concentration after reperfusion,20 rats from the experimental group were sub-divided into four groups:1 ＜ blood sugar ≤ 3 mmol/L ( group C,5 rats ),3 mmol/L ＜ blood sugar ≤ 6 mmol/L ( group D,5 rats),6 mmol/L ≤blood sugar≤9 mmol/L(group E,5 rats),blood sugar ＞9 mmol/L(group F,5 rats) (blood sugar =blood glucose level).TUNEL staining was used to detect the neurons undergoing apoptosis,and Fluoro-Jade (FJB)staining was performed to reveal the degenerating neuronal cell bodies and axons.SAS 8.0 software analysis and processing,staining between the two groups of measurement data using single factor analysis of variance data.Results ( 1 ) TUNEL staining:the percentage of apoptotic neurons showed an obvious increase from C,D,E,and F group ( hippoeampal CA 1:40.2 ± 3.1,38.7 ± 2.4,36.8 ± 2.6 and 76.4 ± 6.3 ; hippocampal DG:62.4± 4.2,59.8 ± 3.7,68.1 ± 2.8 and 125.4 ± 5.8 ) compared to group A and group B ( hippocampal CA1:3.2 ± 1.9,2.8 ± 0.8; hippocampal DG:4.1 ± 2.4,3.4 ± 1.2 ),the difference was statistically significant ( hippocampal CA 1:F =13.52,P ＜ 0.05 ; hippocampal DG:F =14.29,P ＜ 0.05 ) ; among the subgroups from the experimental group,the percentage of apoptotic neurons from group F( hippocampal CA1:76.4 ± 6.3,hippocampal DG:125.4 ± 5.8 ) rose more markedly than the other three groups( group C,D,E,hippocampal CA1:40.2 ±3.1,38.7 ±2.4,36.8 ±2.6 ;hippocampal DG:62.4± 4.2,59.8 ± 3.7,68.1 ± 2.8 ),the difference was statistically significant (hippocampal CA 1:F =5.08,P ＜ 0.05 ; hippocampal DG:F =6.52,P ＜ 0.05 ).(2) FJB staining:there was a statistical significance between group A,B and group C,D,E,F ( hippocampal CA 1:F =18.49,P ＜ 0.05 ; hippocampal DG:F =11.37,P ＜0.05);furthermore,compared with group C,D,E,the percentage of FJB positive cells in group F was significantly increased.,the difference was statistically significant ( hippocampal CA1:F =7.83,P ＜ 0.05 ; hippocampal DG:F =14.29,P ＜ 0.05 ).Conclusion Control the rats on the same level of blood glucose and the same duration of the hypoglycemia,the severity of brain injury is closely correlated to the rising blood glucose concentration after hypoglycemia:the higher glucose level is,the more serious imparement brain suffer.
