CAJ | 학술논문

目的观察特异性阻断Ezrin的表达对人乳腺癌细胞MDA-MB-231和MCF-7的增殖和侵袭能力的影响.方法将anti-pCR3.1-Ezrin质粒经脂质体介导,转染人人乳腺癌细胞MDA-MB-231 6、12和24 h,应用Western blot和逆转录-聚合酶链反应(RT-PCR)方法检测Ezrin的表达变化情况;转染质粒24h后,噻唑蓝(MTT)比色法检测抑制Ezrin对MDA-MB-231和MCF-7细胞体外增殖能力的影响,Boyden小室法检测抑制Ezrin对MDA-MB-231和MCF-7细胞体外侵袭能力的影响.结果转染anti-pCR3.1-Ezrin后,对MDA-MB-231细胞中的Ezrin表达抑制在24 h时达高峰.MTT法比色实验结果显示,MDA-MB-231和MCF-7细胞中转染anti-pCR3.1-Ezrin组、转染空质粒组和对照组的A值分别为0.410±0.018、0.765±0.058、0.795±0.061和0.480±0.021、0.632±0.052、0.648±0.059.转染anti-pCR3.1-Ezrin组细胞的增殖受到明显抑制,抑制率分别为(47.9±3.1)%和(32.0±2.8)%(P<0.05).Boyden小室法检测结果显示,MDA-MB-231和MCF-7细胞中转染anti-pCR3.1-Ezrin组细胞的侵袭能力分别为对照组的(50.5±3.2)%和(74.8±4.6)%(P<0.05).结论 Ezrin在乳腺癌的生长和侵袭过程中发挥重要作用.
목적 관찰특이성조단Ezrin적표체대인유선암세포MDA-MB-231화MCF-7적증식화침습능력적영향.방법 장anti-pCR3.1-Ezrin질립경지질체개도,전염인인유선암세포MDA-MB-231 6、12화24 h,응용Western blot화역전록-취합매련반응(RT-PCR)방법검측Ezrin적표체변화정황;전염질립24h후,새서람(MTT)비색법검측억제Ezrin대MDA-MB-231화MCF-7세포체외증식능력적영향,Boyden소실법검측억제Ezrin대MDA-MB-231화MCF-7세포체외침습능력적영향.결과 전염anti-pCR3.1-Ezrin후,대MDA-MB-231세포중적Ezrin표체억제재24 h시체고봉.MTT법비색실험결과현시,MDA-MB-231화MCF-7세포중전염anti-pCR3.1-Ezrin조、전염공질립조화대조조적A치분별위0.410±0.018、0.765±0.058、0.795±0.061화0.480±0.021、0.632±0.052、0.648±0.059.전염anti-pCR3.1-Ezrin조세포적증식수도명현억제,억제솔분별위(47.9±3.1)%화(32.0±2.8)%(P<0.05).Boyden소실법검측결과현시,MDA-MB-231화MCF-7세포중전염anti-pCR3.1-Ezrin조세포적침습능력분별위대조조적(50.5±3.2)%화(74.8±4.6)%(P<0.05).결론 Ezrin재유선암적생장화침습과정중발휘중요작용.
Objective To observe the influence of proliferation and invasion of human breast cancer ceils MDA-MB-231 after inhibition of Ezrin expression by anti-sense RNA. Methods Human breast cancer cell lines MDA-MB-231 and MCF-7 were transfected with anti-pCR3, 1-Ezrin with lipo-fectamine. Western-blot and RT-PCR were used to detect the expression of Ezrin after transfection for 6, 12,and 24 h,respectively. The proliferative ability of ceils was tested by MTT assay,and the metastatic a-bility of cells was investigated by Boyden cabin after transfection for 24 h. Results After transfection with anti-sense RNA for 24 h,both protein and mRNA expression of Ezrin in MDA-MB-231 cells was signifi-cantly reduced at their peak time. After transfection with anti-sense RNA for 24 h, the MTT assay showed that absorbance (A) values in anti-pCR3, 1-Ezrin,vacuity plasmid and blank control groups were 0.410±0.018,0.765±0.058,0.795±0.061 in MDA-MB-231, and 0.480±0.021,0.632±0.052,0.648±0.059 in MCF-7, respectively. The results suggested that the proliferation of MDA-MB-231 and MCF-7 cells was inhibited significantly in anti-pCR3.1-Ezrin group by (47.9±3.1) % and (32.0±2.8) %, re-spectively. After transfection with anti-sense RNA for 24 h, the invasive ability of MDA-MB-231 and MCF-7 cells was (50.5±3.2) % and (74.8±4.6) % respectively as compared with that of blank control group (P<0.05). Conclusion Ezrin plays an important role in the process of proliferation and invasion of breast cancer cells.