中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2011年
1期
2-5
,共4页
吴冰洁%姜建勇%孙咏虹%岳崴%张玉淼%刘敏%顾平%王铭维
吳冰潔%薑建勇%孫詠虹%嶽崴%張玉淼%劉敏%顧平%王銘維
오빙길%강건용%손영홍%악외%장옥묘%류민%고평%왕명유
运动%快速老化%β-淀粉样蛋白%淀粉样蛋白前体蛋白%阿尔茨海默病
運動%快速老化%β-澱粉樣蛋白%澱粉樣蛋白前體蛋白%阿爾茨海默病
운동%쾌속노화%β-정분양단백%정분양단백전체단백%아이자해묵병
Movement%Senescence%β-Amyloid protein%Amyloid precursor protein%Alzheimer's disease
目的 研究运动对快速老化小鼠(SAMP)8海马β-淀粉样蛋白(Aβ)及淀粉样蛋白前体蛋白(APP)的影响,以探讨运动改善阿尔茨海默病(AD)学习记忆功能的机制.方法 将40只3月龄SAMP8小鼠采用单纯随机抽样法分为运动组和对照组,运动组进行跑笼运动训练,第1个月每周训练5 d,每天10 min,第2个月每周训练5 d,每天20 min.2个月后采用H-E染色观察2组小鼠海马神经元形态改变;免疫组织化学技术检测海马Aβ免疫阳性细胞的表达;逆转录-聚合酶链反应(RT-PCR)技术检测海马APP mRNA的表达水平.结果 对照组5月龄SAMP8小鼠海马部分神经元细胞变性、死亡,核浓缩,空泡变性;运动组偶有神经元细胞变性、死亡,大部分细胞形态正常.运动组海马Aβ免疫阳性细胞表达水平为(0.192±0.018),明显低于对照组的(0.274±0.017)(P＜0.05);运动组海马APP mRNA表达水平为(0.168±0.059),明显低于对照组的(0.574±0.115)(P＜0.01).结论 运动可以延缓SAMP8小鼠海马神经元变性,降低海马Aβ及APP的表达,这可能是运动改善AD学习记忆功能的重要机制之一.
目的 研究運動對快速老化小鼠(SAMP)8海馬β-澱粉樣蛋白(Aβ)及澱粉樣蛋白前體蛋白(APP)的影響,以探討運動改善阿爾茨海默病(AD)學習記憶功能的機製.方法 將40隻3月齡SAMP8小鼠採用單純隨機抽樣法分為運動組和對照組,運動組進行跑籠運動訓練,第1箇月每週訓練5 d,每天10 min,第2箇月每週訓練5 d,每天20 min.2箇月後採用H-E染色觀察2組小鼠海馬神經元形態改變;免疫組織化學技術檢測海馬Aβ免疫暘性細胞的錶達;逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測海馬APP mRNA的錶達水平.結果 對照組5月齡SAMP8小鼠海馬部分神經元細胞變性、死亡,覈濃縮,空泡變性;運動組偶有神經元細胞變性、死亡,大部分細胞形態正常.運動組海馬Aβ免疫暘性細胞錶達水平為(0.192±0.018),明顯低于對照組的(0.274±0.017)(P＜0.05);運動組海馬APP mRNA錶達水平為(0.168±0.059),明顯低于對照組的(0.574±0.115)(P＜0.01).結論 運動可以延緩SAMP8小鼠海馬神經元變性,降低海馬Aβ及APP的錶達,這可能是運動改善AD學習記憶功能的重要機製之一.
목적 연구운동대쾌속노화소서(SAMP)8해마β-정분양단백(Aβ)급정분양단백전체단백(APP)적영향,이탐토운동개선아이자해묵병(AD)학습기억공능적궤제.방법 장40지3월령SAMP8소서채용단순수궤추양법분위운동조화대조조,운동조진행포롱운동훈련,제1개월매주훈련5 d,매천10 min,제2개월매주훈련5 d,매천20 min.2개월후채용H-E염색관찰2조소서해마신경원형태개변;면역조직화학기술검측해마Aβ면역양성세포적표체;역전록-취합매련반응(RT-PCR)기술검측해마APP mRNA적표체수평.결과 대조조5월령SAMP8소서해마부분신경원세포변성、사망,핵농축,공포변성;운동조우유신경원세포변성、사망,대부분세포형태정상.운동조해마Aβ면역양성세포표체수평위(0.192±0.018),명현저우대조조적(0.274±0.017)(P＜0.05);운동조해마APP mRNA표체수평위(0.168±0.059),명현저우대조조적(0.574±0.115)(P＜0.01).결론 운동가이연완SAMP8소서해마신경원변성,강저해마Aβ급APP적표체,저가능시운동개선AD학습기억공능적중요궤제지일.
Objective To explore the effects of movement on hippocampal β-amyloid protein ( Aβ ) and amyloid precursor protein (APP) in senescence-accelerated and senescence-prone (SAMP8) mice, and the mechanism by which movement improves learning and memory in mice with a model of Alzheimer's disease. Methods Forty 3-month-old SAMP8 mice were divided randomly into a movement group and a control group. The movement group was trained with a running wheel 10 min daily, 5 days a week in the first month, and 20 min daily in the second month. Morphological changes in the hippocampus were observed under the microscope after HE staining. The expression of Aβ in the hippocampus was detected by immumohistochemical methods and APP mRNA expression was detected by RT-PCR two months later. Results HE staining showed neuron degeneration and death, chromatin condensation and vacuolar degeneration in the hippocampus of the 5-mouth-old SAMP8 mice of the control group. The movement group showed less neuron degeneration and death, and the morphology of most cells was normal The expression of Aβ in the hippocampus of the 5-month-old SAMP8 mice in the movement group was significantly lower than that in the control group. APP mRNA expression levels in the movement group were also significantly lower.Conclusions Movement can delay neuron degeneration and down-regulate Aβ and APP mRNA expression levels in the hippocampus of SAMP8 mice. It may be an important mechanism by which movement improves learning and memory in mice with a model of Alzheimer's disease.
