CAJ | 학술논문

目的探讨不同功率2450MHz微波辐射不同时间对原代培养的小鼠成纤维细胞细胞外调节激酶-1/2(ERK-1/2)活化的影响.方法分离纯化培养小鼠成纤维细胞,经细胞形态学观察及鉴定后,采用2450 MHz微波直接辐射小鼠成纤维细胞,输出功率分别为0.3 W/cm2、0.5 W/cm2、1 W/cm2,每组功率辐射时间分别为15 min和30 min.采用Western-blot方法分析微波辐射后成纤维细胞内丝裂原激活蛋白激酶(MAPK)信号通路激活状态.结果输出功率0.3 W/cm2、0.5 W/cm2和1 W/cm2辐射15 min时ERK-1/2磷酸化程度明显增强;随着辐射时间延长.ERK-1/2磷酸化程度有下降趋势.结论体外不同功率微波短时间辐射能激活小鼠成纤维细胞的MAPK信号传导通路,长时间辐射能抑制MAPK信号传导通路,提示不同的微波辐射功率及时间对小鼠成纤维细胞信号传导有着不同的影响.
목적 탐토불동공솔2450MHz미파복사불동시간대원대배양적소서성섬유세포세포외조절격매-1/2(ERK-1/2)활화적영향.방법 분리순화배양소서성섬유세포,경세포형태학관찰급감정후,채용2450 MHz미파직접복사소서성섬유세포,수출공솔분별위0.3 W/cm2、0.5 W/cm2、1 W/cm2,매조공솔복사시간분별위15 min화30 min.채용Western-blot방법분석미파복사후성섬유세포내사렬원격활단백격매(MAPK)신호통로격활상태.결과 수출공솔0.3 W/cm2、0.5 W/cm2화1 W/cm2복사15 min시ERK-1/2린산화정도명현증강;수착복사시간연장.ERK-1/2린산화정도유하강추세.결론 체외불동공솔미파단시간복사능격활소서성섬유세포적MAPK신호전도통로,장시간복사능억제MAPK신호전도통로,제시불동적미파복사공솔급시간대소서성섬유세포신호전도유착불동적영향.
Objective To investigate the effects of different intensities and different radiation durations of 2450MHz microwaves on activation of extracellular-regulated kinase (ERK)-1/2 in cultured mouse fibroblast in vitro.Methods The fibroblasts isolated from mouse skin and cultured were directly radiated with different intensities and different durations of microwave.After direct radiation with 2450MHz microwave (0.3 W/cm2, 0.5W/cm2, 1W/cm2 ) for 15min or 30min, anti-phosphorylation extracellular-regulated kinase( ERK-1/2 ) antibody was analyzed by Western blot technique to observe phosphorylation changes of fibroblasts protein ERK-1/2.and activation of mitogen-activated progein kinase(MAPK) signal conductive pathways.Results After microwave radiation with 0.3W/cm2, 0.5W/cm2, 1W/cm2for 15min, activation of ERK-1/2 increased significantly (P < 0.05 ) ; but after 30min radiation with 0.3 W/cm2,0.5W/cm2, 1W/cm2, activation of ERK-1/2 decreased significantly(P<0.05).Conclusion It is concluded that different intensities microwave may activate different signals of fibroblasts in dose-dependent manner in vitro.Different microwave radiations can induce different activations in MAPK signal conductive pathways.