中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2009年
1期
21-23
,共3页
童永喜%李兰娟%娄国强%张永乐%赵念博
童永喜%李蘭娟%婁國彊%張永樂%趙唸博
동영희%리란연%루국강%장영악%조념박
肝炎病毒,乙型%原发性肝癌%基本核心启动子%前C区基因%变异
肝炎病毒,乙型%原髮性肝癌%基本覈心啟動子%前C區基因%變異
간염병독,을형%원발성간암%기본핵심계동자%전C구기인%변이
Hepatitis B virus%Primary liver cancer%Basic core promoter%Preeore gene%Mutation
目的 研究原发性肝癌(PLC)与HBV基本核心启动子(BCP)和前C区基因变异的相关性.方法 对144例HBsAg(+)PLC患者的血清进行HBV标志物和HBV DNA检测,对HBeAg(-)但HBV DNA(+)的样本再采用实时荧光定量PCR进行前C区和BCP区基因变异检测.随机选取120例慢性乙型肝炎(CHB)患者作为对照组.结果 PLC患者血清HBV标志物中HBeAg(+)46例.占31.94%,HBeAg(-)98例,占68.06%.在98例HBeAg(-)患者的血清中,HBV DNA(+)56例,占57.14%,其中前C1896变异43例,占76.79%,BCP1762/1764基因变异50例,占89.29%,共同变异38例,占67.86%.PLC患者中的前C1896和BCP1762/1764基因变异率高于CHB患者,且差异有统计学意义(χ2值分别为9.36和5.77,P值均＜0.05).结论 PLC的发生可能与前C区和BCP区基凶变异有关.
目的 研究原髮性肝癌(PLC)與HBV基本覈心啟動子(BCP)和前C區基因變異的相關性.方法 對144例HBsAg(+)PLC患者的血清進行HBV標誌物和HBV DNA檢測,對HBeAg(-)但HBV DNA(+)的樣本再採用實時熒光定量PCR進行前C區和BCP區基因變異檢測.隨機選取120例慢性乙型肝炎(CHB)患者作為對照組.結果 PLC患者血清HBV標誌物中HBeAg(+)46例.佔31.94%,HBeAg(-)98例,佔68.06%.在98例HBeAg(-)患者的血清中,HBV DNA(+)56例,佔57.14%,其中前C1896變異43例,佔76.79%,BCP1762/1764基因變異50例,佔89.29%,共同變異38例,佔67.86%.PLC患者中的前C1896和BCP1762/1764基因變異率高于CHB患者,且差異有統計學意義(χ2值分彆為9.36和5.77,P值均＜0.05).結論 PLC的髮生可能與前C區和BCP區基兇變異有關.
목적 연구원발성간암(PLC)여HBV기본핵심계동자(BCP)화전C구기인변이적상관성.방법 대144례HBsAg(+)PLC환자적혈청진행HBV표지물화HBV DNA검측,대HBeAg(-)단HBV DNA(+)적양본재채용실시형광정량PCR진행전C구화BCP구기인변이검측.수궤선취120례만성을형간염(CHB)환자작위대조조.결과 PLC환자혈청HBV표지물중HBeAg(+)46례.점31.94%,HBeAg(-)98례,점68.06%.재98례HBeAg(-)환자적혈청중,HBV DNA(+)56례,점57.14%,기중전C1896변이43례,점76.79%,BCP1762/1764기인변이50례,점89.29%,공동변이38례,점67.86%.PLC환자중적전C1896화BCP1762/1764기인변이솔고우CHB환자,차차이유통계학의의(χ2치분별위9.36화5.77,P치균＜0.05).결론 PLC적발생가능여전C구화BCP구기흉변이유관.
Objective To investigate the association of primary liver cancer(PLC)with the mutations of HBV precore and basic core promoter(BCP)genes.Methods The serum markers of hepatitis B and the quantities of serum HBV DNA were detected in 144 HBsAg-positive PLC patients.The precore and BCP gene mutations in patients with HBeAg-negtive and HBV DNA-positive were detected by real-time PCR.One hundred and twenty chronic hepatitis B(CHB)patients were randomly selected to serve as the conol.Results There were 46(3 1.94%)patients with HBeAg-positive and 98(68.06%)patients with HBeAg-negative.In 98 HBeAg-negative patients,56(57.14%)were HBV DNA-positive,in which 43 (76.79%)were with precore 1896 gene mutations,50(89.29%)were with BCP1762/1764 gene mutations.and 38(67.86%)were with both gene mutations.Precore 1896 and BCP1762/1764 gene mutation rates in PLC patients were much higher than those in CHB patients(χ2=9.36 and 5.77,P＜0.05).Conclusion PLC may be associated with the mutations of HBV precore anti BCP genes.
