中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2010年
10期
773-777
,共5页
脂肪肝%环氧合酶-2%多态性,单核苷酸
脂肪肝%環氧閤酶-2%多態性,單覈苷痠
지방간%배양합매-2%다태성,단핵감산
Fatty liver%,Cyclooxygenase-2%Polymorphism,single nucleotide
目的 通过检测环氧合酶-2(COX-2)基因启动子区单核苷酸的多态性,以探讨其与非酒精性脂肪肝(NAFLD)遗传易感性的关系.方法 对200例NAFLD患者和206名正常对照,采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法对COX-2基因启动子区-765G＞C和-1195G＞A多态性进行基因型分析.计量资料结果用均数±标准差((-x)±s)表示,经方差齐性检验后,行t检验;性别、基因型及等位基因频率的比较行x2检验.结果 正常对照中,COX-2基因启动子区-765G＞C和-1195G＞A等位基因的分布频率分别为48%和2%,NAFLD患者组二者分别为54%和5%.多变量Logistic回归分析显示:-765GC基因携带者与-765GG基因携带者相比较,前者发生NAFLD的比值比(OR)=2.35 (95% CI为1.17～3.65);-1195AA基因携带者与-1195GG基因携带者相比较,前者发生NAFLD的OR=1.13 (95% CI为1.01～2.46).与单体型G-1195-G765相比较,含有A1195的A-1195-C-765、A1195-G765两种单体型发生NAFLD的相对风险明显升高,OR分别为1.42 (95% CI为1.11～1.63,P＜0.05)和4.24(95% CI为1.72～14.22,P＜0.01).且A-1195-C765发生NAFLD的OR值高于A-1195-G-765、G-1195-C765的单体型.这一结果提示在同一单体型内-1195A与-765C之间存在交互作用.结论 COX-2基因启动子区的-1195G＞A和-765G＞C单核苷酸存在多态性,与NAFLD发生相关,是决定NAFLD个体遗传易感性的重要因素.
目的 通過檢測環氧閤酶-2(COX-2)基因啟動子區單覈苷痠的多態性,以探討其與非酒精性脂肪肝(NAFLD)遺傳易感性的關繫.方法 對200例NAFLD患者和206名正常對照,採用多聚酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法對COX-2基因啟動子區-765G＞C和-1195G＞A多態性進行基因型分析.計量資料結果用均數±標準差((-x)±s)錶示,經方差齊性檢驗後,行t檢驗;性彆、基因型及等位基因頻率的比較行x2檢驗.結果 正常對照中,COX-2基因啟動子區-765G＞C和-1195G＞A等位基因的分佈頻率分彆為48%和2%,NAFLD患者組二者分彆為54%和5%.多變量Logistic迴歸分析顯示:-765GC基因攜帶者與-765GG基因攜帶者相比較,前者髮生NAFLD的比值比(OR)=2.35 (95% CI為1.17～3.65);-1195AA基因攜帶者與-1195GG基因攜帶者相比較,前者髮生NAFLD的OR=1.13 (95% CI為1.01～2.46).與單體型G-1195-G765相比較,含有A1195的A-1195-C-765、A1195-G765兩種單體型髮生NAFLD的相對風險明顯升高,OR分彆為1.42 (95% CI為1.11～1.63,P＜0.05)和4.24(95% CI為1.72～14.22,P＜0.01).且A-1195-C765髮生NAFLD的OR值高于A-1195-G-765、G-1195-C765的單體型.這一結果提示在同一單體型內-1195A與-765C之間存在交互作用.結論 COX-2基因啟動子區的-1195G＞A和-765G＞C單覈苷痠存在多態性,與NAFLD髮生相關,是決定NAFLD箇體遺傳易感性的重要因素.
목적 통과검측배양합매-2(COX-2)기인계동자구단핵감산적다태성,이탐토기여비주정성지방간(NAFLD)유전역감성적관계.방법 대200례NAFLD환자화206명정상대조,채용다취매련반응-한제성편단장도다태성(PCR-RFLP)방법대COX-2기인계동자구-765G＞C화-1195G＞A다태성진행기인형분석.계량자료결과용균수±표준차((-x)±s)표시,경방차제성검험후,행t검험;성별、기인형급등위기인빈솔적비교행x2검험.결과 정상대조중,COX-2기인계동자구-765G＞C화-1195G＞A등위기인적분포빈솔분별위48%화2%,NAFLD환자조이자분별위54%화5%.다변량Logistic회귀분석현시:-765GC기인휴대자여-765GG기인휴대자상비교,전자발생NAFLD적비치비(OR)=2.35 (95% CI위1.17～3.65);-1195AA기인휴대자여-1195GG기인휴대자상비교,전자발생NAFLD적OR=1.13 (95% CI위1.01～2.46).여단체형G-1195-G765상비교,함유A1195적A-1195-C-765、A1195-G765량충단체형발생NAFLD적상대풍험명현승고,OR분별위1.42 (95% CI위1.11～1.63,P＜0.05)화4.24(95% CI위1.72～14.22,P＜0.01).차A-1195-C765발생NAFLD적OR치고우A-1195-G-765、G-1195-C765적단체형.저일결과제시재동일단체형내-1195A여-765C지간존재교호작용.결론 COX-2기인계동자구적-1195G＞A화-765G＞C단핵감산존재다태성,여NAFLD발생상관,시결정NAFLD개체유전역감성적중요인소.
Objective to study the relationship between COX-2 gene and hereditariness to Nonalcoholic fatty liver disease by detecting single nucleotide polymorphisms in the promoter of COX-2 gene. Methods Genotypes of 200 case patients with NAFLD and 206 control subjects were examined by polymerase chain reaction-based restriction fragment length polymorphism(PCR-RFLP). The DNA samples were extracted from the peripheral blood of all subjects. Results Two SNPs, -1195G＞A and -765 G＞C, were indentified with frequencies of variant alleles 54% and 5% in patients with NAFLD and 48% and 2% in control, respectively. A case-control analysis revealed a 1.13-fold (95% CI = 1.01-2.46) and a 2.35-fold (95% CI = 1.17-3.65) excess risk of developing NAFLD for -1195AA or -765CG genotype carriers compared with noncarriers. Compared with G-1195-G-765 containing haplotype, a greater risk of developing NAFLD was observed for A-1195-G-765 (OR =1.42; 95% CI =1.11-1.63) and A-1195-C-765 (OR = 4.24; 95% CI =1.72-14.22)containing haplotypes. A greater risk of developing NAFLD was observed for A-1195 and C-765 containing haplotype compared with other haplotype, suggesting an interaction between the -1195A and -765C in the context of haplotype. Conclusion These findings suggest that genetic variants in the COX-2 promoter may play an important role in mediating susceptibility to developing NAFLD in a Chinese population. -1195G＞A and -765G＞C in promoter region of Cyclooxygenase-2 gene, whose single nucleotide polymorphisms are related with development of NAFLD, are the significance factors of the susceptibility of NAFLD.
