CAJ | 학술논문

目的探讨Leptin对人单核细胞协同刺激分子和HLA-DR表达的影响,为阐明瘦素在抗感染免疫中的作用提供依据.方法采用流式细胞术检测THP-1细胞和人外周血单核细胞表面CD86和HLA-DR的表达变化.结果经高浓度瘦素处理后,THP-1细胞CD86和HLA-DR的表达明显增强(CD86未处理组:8.78±1.66,CD86leptin10:50.76±4.29,CD86leptin100:95.20±4.90；HLA未处理组:20.75±2.12,HLAleptin10:102.14±5.75,HLAleptin100:104.32±4.75).人外周血单核细胞表面的CD86和HLA-DR的表达也出现了类似增加的现象(CD86未处理组:17.91±1.78,CD86leptin100:48.80±3.60;HLA未处理组:34.10±2.76,HLAleptin100:88.86 ±3.53).结论瘦素可以上调人单核细胞表面协同刺激分子和MHC-Ⅱ类分子的表达,从而增强单核细胞的抗原提呈功能.
목적 탐토Leptin대인단핵세포협동자격분자화HLA-DR표체적영향,위천명수소재항감염면역중적작용제공의거.방법 채용류식세포술검측THP-1세포화인외주혈단핵세포표면CD86화HLA-DR적표체변화.결과 경고농도수소처리후,THP-1세포CD86화HLA-DR적표체명현증강(CD86미처리조:8.78±1.66,CD86leptin10:50.76±4.29,CD86leptin100:95.20±4.90；HLA미처리조:20.75±2.12,HLAleptin10:102.14±5.75,HLAleptin100:104.32±4.75).인외주혈단핵세포표면적CD86화HLA-DR적표체야출현료유사증가적현상(CD86미처리조:17.91±1.78,CD86leptin100:48.80±3.60;HLA미처리조:34.10±2.76,HLAleptin100:88.86 ±3.53).결론 수소가이상조인단핵세포표면협동자격분자화MHC-Ⅱ류분자적표체,종이증강단핵세포적항원제정공능.
Objective To study the effects of Leptin on the expression of CD86 and HLA-DR in human monocytes.Methods The expression of CD86 and HLA-DR in THP-1 Cells and human primary monocytes were detected by flow cytometry.Results Expression of CD86 and HLA-DR in THP-1 cells was significantly increased after treatment with high-dose Leptin ( CD86Untreated group:8.78 ± 1.66,CD86leptin10:50.76 ± 4.29,CD86leptin100:95.20 ± 4.90; HLAUntreated group:20.75 ± 2.12,HLAleptin10:102.14 ± 5.75,HLAleptin100:104.32 ± 4.75;).The similar results were observed in human primary monocytes ( CD86Untreated group:17.91 ± 1.78,CD86leptin100:48.80 ± 3.60; HLAUntreated group:34.10 ± 2.76,HLAleptin100:88.86 ± 3.53).Conclusions By up-regulating CD86 and HLA-DR expression,Leptin might enhance the ability to present antigen in THP - 1 cells and human monocytes.