CAJ | 학술논문

目的探讨鞘内给予细胞周期依赖性激酶5(cyclin-dependent kinases,Cdk5)特异性拮抗剂Roscovitine对小鼠骨癌痛行为学的影响.方法 24只C3H/Hej 小鼠采用随机数字表法随机分为3组,S组(假手术后14 d+溶媒)、C组(接种后14d+溶媒)、R组(接种后14 d+Roscovitine),每组8只.C组和R组将含2×105个纤维肉瘤NCTC 2472细胞的最小必需培养基(α-MEM)20μl注射到小鼠右侧股骨远端骨髓腔内,制作骨癌痛模型.S组只注入不含肿瘤细胞的α-MEM.术后14 d,R组小鼠鞘内注射含有20μg Roscovitine的二甲基亚砜(DMSO)5μl,C组及S组小鼠鞘内注DMSO 5 μl.观察给药前及给药后1、6、24、48、72 h检测小鼠痛行为学的变化:机械缩足阈值(paw uithdrawal mechanical threshold,PMWT)和热缩足潜伏期(Paw withdrawal thermal latency,PWTL).结果各组术前基础机械缩足阈值及热缩足潜伏期比较差异无统计学意义(P＞0.05).接种后7 d,C组PMWT(1.08±0.24)g,与S组(1.85±0.28)g相比明显降低(P＜0.05);接种后10 d,C组PTWL(12.7±1.4)s较S组(18.6±2.1)s明显缩短(P＜0.05),C组小鼠的痛行为学随时间逐渐加重,与S组小鼠比较差异有统计学意义(P＜0.05);与C组及给药前基础值相比,鞘内注射Roscovitine 20μg后6 h PMWT(0.70±0.19)g显著增加(P＜0.05),PTWL(14.16±1.07)s显著延长(P＜0.05),并随时间逐渐增加,12 h达最大值,后逐渐降低,72 h降低到C组水平.结论鞘内注射Roscovitine可有效缓解小鼠骨癌痛.
목적 탐토초내급여세포주기의뢰성격매5(cyclin-dependent kinases,Cdk5)특이성길항제Roscovitine대소서골암통행위학적영향.방법 24지C3H/Hej 소서채용수궤수자표법수궤분위3조,S조(가수술후14 d+용매)、C조(접충후14d+용매)、R조(접충후14 d+Roscovitine),매조8지.C조화R조장함2×105개섬유육류NCTC 2472세포적최소필수배양기(α-MEM)20μl주사도소서우측고골원단골수강내,제작골암통모형.S조지주입불함종류세포적α-MEM.술후14 d,R조소서초내주사함유20μg Roscovitine적이갑기아풍(DMSO)5μl,C조급S조소서초내주DMSO 5 μl.관찰급약전급급약후1、6、24、48、72 h검측소서통행위학적변화:궤계축족역치(paw uithdrawal mechanical threshold,PMWT)화열축족잠복기(Paw withdrawal thermal latency,PWTL).결과 각조술전기출궤계축족역치급열축족잠복기비교차이무통계학의의(P＞0.05).접충후7 d,C조PMWT(1.08±0.24)g,여S조(1.85±0.28)g상비명현강저(P＜0.05);접충후10 d,C조PTWL(12.7±1.4)s교S조(18.6±2.1)s명현축단(P＜0.05),C조소서적통행위학수시간축점가중,여S조소서비교차이유통계학의의(P＜0.05);여C조급급약전기출치상비,초내주사Roscovitine 20μg후6 h PMWT(0.70±0.19)g현저증가(P＜0.05),PTWL(14.16±1.07)s현저연장(P＜0.05),병수시간축점증가,12 h체최대치,후축점강저,72 h강저도C조수평.결론 초내주사Roscovitine가유효완해소서골암통.
Objective To investigate the influences of intrathecal administration of roscovitine on bone cancer pain in mouse models. Methods Twenty four C3H/Hej mice were divided randomly into group S(sham + vehicle ), group C(tumor + vehicle) or group R (tumor+ roscovitine) (n = 8 ). Osteosarcoma NCTC 2472 cells were implanted into the intramedullary space of the right femurs of mice to make bone cancer pain models. α-MEM (group Sand group C) or roscovitine (group R) was injected in 14 post-implanted day. Pain behaviors such as paw withdrawal mechanical threshold(PMWT) and paw withdrawal thermal latency (PWTL) were observed at 1,6, 24,48 and 72 post-injective hour, respectively. Results The level of PMWT and PTWL among every group had no difference before operation. At day 7 after inoculation, PMWT of group tumor decreased to (1.08±0.24) g, and at day 10 PTWL shortened to (12.7±1.4) s, and then the pain behaviors of group tumor showed aggravation along with the development of cancer pain. There was significant difference in the pain behavior thresholds between group sham and group tumor (P＜0.05). The level of PM WT [ (0.70±0.19 ) g ] and PTWL [ ( 14.16±1.07 ) s] increased compared with group C and base level at 6 h after intrathecal administration of 20 μg Roscovitine, and kept increasing until reaching maximum level after 12 h, then the value decreased gradually, lowering to the level of group C after 72 h. Conclusion Intrathecal administration of roscovitine can relief bone cancer pain in mouse models.