CAJ | 학술논문

目的该研究旨在观察人肝细胞癌(hepatocellular carcinoma,HCC)组织中CD146与VEGF的表达及其关系,探讨它们在HCC中与血管生成的关系及其临床意义.方法采用免疫组化法检测60例HCC癌组织及癌旁肝组织中CD146、VEGF的表达情况,用CD34标记免疫组化法检测微血管密度(microvessel density,MVD),分析它们的表达与临床病理指标的关系.结果 CD146、VEGF在癌组织中的阳性率分别为66.67%和63.33%,而在癌旁组织中的阳性率分别为30%和33.33%,癌组织与癌旁组织比较差异有显著性(P＜0.05).癌组织的MVD为54.92±8.55/200倍视野,癌旁组织的MVD为21.36±6.63/200倍视野,两者差异有显著性(P＜0.05).与癌旁组织相比,癌组织中CD146、VEGF表达及MVD明显增加.CD146在人肝癌组织中的表达与临床分期、门静脉癌栓及肝外转移明显有关,而与术后复发、肿瘤个数、肿瘤直径、血清AFP水平及肿瘤分化程度无明显关系.VEGF在人肝癌组织中的表达与术后复发、肝外转移、临床分期、门静脉癌栓、肿瘤直径相关,而与肿瘤个数、血清AFP水平及肿瘤分化程度无明显关系.在癌组织中MVD与CD146、VEGF的表达呈正相关,CD146与VEGF亦呈正相关.结论肝癌组织中CD146及VEGF高表达,与肿瘤血管形成和转移密切相关,分析它们的表达有助于综合判断HCC的预后.
목적 해연구지재관찰인간세포암(hepatocellular carcinoma,HCC)조직중CD146여VEGF적표체급기관계,탐토타문재HCC중여혈관생성적관계급기림상의의.방법 채용면역조화법검측60례HCC암조직급암방간조직중CD146、VEGF적표체정황,용CD34표기면역조화법검측미혈관밀도(microvessel density,MVD),분석타문적표체여림상병리지표적관계.결과 CD146、VEGF재암조직중적양성솔분별위66.67%화63.33%,이재암방조직중적양성솔분별위30%화33.33%,암조직여암방조직비교차이유현저성(P＜0.05).암조직적MVD위54.92±8.55/200배시야,암방조직적MVD위21.36±6.63/200배시야,량자차이유현저성(P＜0.05).여암방조직상비,암조직중CD146、VEGF표체급MVD명현증가.CD146재인간암조직중적표체여림상분기、문정맥암전급간외전이명현유관,이여술후복발、종류개수、종류직경、혈청AFP수평급종류분화정도무명현관계.VEGF재인간암조직중적표체여술후복발、간외전이、림상분기、문정맥암전、종류직경상관,이여종류개수、혈청AFP수평급종류분화정도무명현관계.재암조직중MVD여CD146、VEGF적표체정정상관,CD146여VEGF역정정상관.결론 간암조직중CD146급VEGF고표체,여종류혈관형성화전이밀절상관,분석타문적표체유조우종합판단HCC적예후.
Objective To investigate the expression and clinical significance of CD146 and vascular endothelial growth factor(VEGF)in human hepatocellular carcinoma(HCC)tissues and explore their correlation with angiogenesis of HCC.Methods Immunohistochemistry was employed to determine the expression of CD146 and VEGF and microvessel density(MVD)in HCC tissues and their adjacent liver tissues from 60 cases.Results The positive rates of CD146 and VEGF were significantly higher in the HCC tissues than in the adjacent liver tissues(66.67% vs.30% and 63.33% vs.33.33%,P＜0.05).MVD was markedly higher in the HCC tissue than in the adjacent liver tissue (54.92±8.55 vs.21.36±6.63,P＜0.05).The expression of CD146 in the HCC tissue was significantly correlated with the clinical stage,the portal vein cancer embolism and the presence of extrahepatic metastasis but not with the recurrence of tumor,the number of tumor,the size of tumor,the level of alpha-fetoprotein(AFP)in serum and the differentiation degree of tumor.The expression of VEGF in the HCC tissue was significantly correlated with the recurrence of tumor, the presence of extrahepatic metastasis,the clinical stage,the portal vein cancer embolism and the size of tumor but not with the number of tumor,the level of alpha-fetoprotein(AFP)in serum and the differentiation degree of tumor.MVD was positively correlated with expressions of CD146 and VEGF.The expression of CD146 was positively correlated with that of VEGF.Conclusion The aberrant expression of CD146 and VEGF in HCC tissues is closely correlated to the angiogenesis and metastasis of HCC and analyzing their expression may be helpful for evaluating the prognosis of HCC.