中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2011年
7期
496-500
,共5页
念珠菌病,外阴阴道%肠疾病%念珠菌病%带病原状态%复发
唸珠菌病,外陰陰道%腸疾病%唸珠菌病%帶病原狀態%複髮
념주균병,외음음도%장질병%념주균병%대병원상태%복발
Candidiasis,vulvovaginal%Intestinal diseases%Candidiasis%Carrier state%Recurrence
目的 采用微生物学和分子生物学方法,研究外阴阴道假丝酵母菌病患者肠道假丝酵母菌携带与阴道假丝酵母菌感染之间的关系.方法 收集2006年4月至2008年2月问上海市长宁区中心医院就诊的148例外阴阴道假丝酵母菌病患者的阴道分泌物和肛拭子标本进行假丝酵母菌的培养和鉴定.采用内转录间隔区(ITS)PCR和随机扩增多态性DNA(RAPD)PCR方法 对两处假丝酵母菌基因组DNA进行比对和分型,从而确定其关系.比较合并肠道携带假丝酵母菌的外阴阴道假丝酵母菌病患者与单纯阴道感染假丝酵母菌但肠道内无假丝酵母菌携带者阴道局部使用抗真菌药物治疗后复发率的差异.结果 148例外阴阴道假丝酵母菌患者中,白假丝酵母菌感染占绝大多数(91.9%,136/148);33.1%(49/148)的患者合并肠道携带假丝酵母菌.ITS PCR结果 显示,92%(22/24)的患者肠道与阴道假丝酵母菌菌株间有高度同源性.患者在接受阴道局部抗真菌治疗后,合并肠道携带假丝酵母菌的外阴阴道假丝酵母菌病患者,其复发率(7/14)高于单纯的阴道感染者[复发率为21%(6/29)],两者比较,差异有统计学意义(P<0.05).结论 肠道携带假丝酵母菌与外阴阴道假丝酵母菌病之间存在着一定的关系.外阴阴道假丝酵母菌病患者在合并肠道携带假丝酵母菌时,阴道局部治疗后的外阴阴道假丝酵母菌病复发率较高.
目的 採用微生物學和分子生物學方法,研究外陰陰道假絲酵母菌病患者腸道假絲酵母菌攜帶與陰道假絲酵母菌感染之間的關繫.方法 收集2006年4月至2008年2月問上海市長寧區中心醫院就診的148例外陰陰道假絲酵母菌病患者的陰道分泌物和肛拭子標本進行假絲酵母菌的培養和鑒定.採用內轉錄間隔區(ITS)PCR和隨機擴增多態性DNA(RAPD)PCR方法 對兩處假絲酵母菌基因組DNA進行比對和分型,從而確定其關繫.比較閤併腸道攜帶假絲酵母菌的外陰陰道假絲酵母菌病患者與單純陰道感染假絲酵母菌但腸道內無假絲酵母菌攜帶者陰道跼部使用抗真菌藥物治療後複髮率的差異.結果 148例外陰陰道假絲酵母菌患者中,白假絲酵母菌感染佔絕大多數(91.9%,136/148);33.1%(49/148)的患者閤併腸道攜帶假絲酵母菌.ITS PCR結果 顯示,92%(22/24)的患者腸道與陰道假絲酵母菌菌株間有高度同源性.患者在接受陰道跼部抗真菌治療後,閤併腸道攜帶假絲酵母菌的外陰陰道假絲酵母菌病患者,其複髮率(7/14)高于單純的陰道感染者[複髮率為21%(6/29)],兩者比較,差異有統計學意義(P<0.05).結論 腸道攜帶假絲酵母菌與外陰陰道假絲酵母菌病之間存在著一定的關繫.外陰陰道假絲酵母菌病患者在閤併腸道攜帶假絲酵母菌時,陰道跼部治療後的外陰陰道假絲酵母菌病複髮率較高.
목적 채용미생물학화분자생물학방법,연구외음음도가사효모균병환자장도가사효모균휴대여음도가사효모균감염지간적관계.방법 수집2006년4월지2008년2월문상해시장저구중심의원취진적148예외음음도가사효모균병환자적음도분비물화항식자표본진행가사효모균적배양화감정.채용내전록간격구(ITS)PCR화수궤확증다태성DNA(RAPD)PCR방법 대량처가사효모균기인조DNA진행비대화분형,종이학정기관계.비교합병장도휴대가사효모균적외음음도가사효모균병환자여단순음도감염가사효모균단장도내무가사효모균휴대자음도국부사용항진균약물치료후복발솔적차이.결과 148예외음음도가사효모균환자중,백가사효모균감염점절대다수(91.9%,136/148);33.1%(49/148)적환자합병장도휴대가사효모균.ITS PCR결과 현시,92%(22/24)적환자장도여음도가사효모균균주간유고도동원성.환자재접수음도국부항진균치료후,합병장도휴대가사효모균적외음음도가사효모균병환자,기복발솔(7/14)고우단순적음도감염자[복발솔위21%(6/29)],량자비교,차이유통계학의의(P<0.05).결론 장도휴대가사효모균여외음음도가사효모균병지간존재착일정적관계.외음음도가사효모균병환자재합병장도휴대가사효모균시,음도국부치료후적외음음도가사효모균병복발솔교고.
Objective To investigate the relationship between vaginal and intestinal Candida in patients with vulvovaginal candidiasis by using microbiological and molecular methods. Methods The samples of vaginal discharge and anal swabs were collected from 148 cases with vulvovaginal candidiasis,followed by fungal culture, identification, purification and genome DNA extraction. The genome sequences from respective locations were aligned and typed according to their homology analyzed by internal transcribed spacer (ITS) PCR and random amplified polymorphic DNA (RAPD) PCR. Patients with vulvovaginal infection or those with infections in intestine and vulvovagina were pooled respectively, while the recurrent incidences after local anti-fungal treatments were analyzed. Results Candida albicans is the dominant pathogen in 148 cases with vulvovaginal candidiasis (91.9% , 136/148) ; 33. 1% (49/148) of patients with vulvovaginal candidiasis were infected in both intestine and vulvovagina. While 92% (22/24) of patients with intestinal and vaginal Candida infection showed high homology. The recurrent rate of patients with vulvovaginal candidiasis complicated with concurrent intestinal Candida infection (7/14) was significantly higher than that of solo vaginal infected patients [21% (6/29)] after vaginal treatment (P < 0. 05) . Conclusions The infection of vulvovaginal candidiasis is highly associated with the concurrent infection of intestinal Candida. The recurrent rate is high in patients with vulvovaginal candidiasis with concurrent infection of intestinal Candida after vaginal treatment. The general management to those patients infected by both vulvovaginal and intestinal Candida is necessary in reducing the recurrence of the disease.
