中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2010年
3期
242-246
,共5页
张长成%张大伟%荆小伟%赵培冉%金丹%魏宽海%裴国献
張長成%張大偉%荊小偉%趙培冉%金丹%魏寬海%裴國獻
장장성%장대위%형소위%조배염%금단%위관해%배국헌
组织工程%新生血管化%生理性%骨形态发生蛋白质类%引导骨再生技术
組織工程%新生血管化%生理性%骨形態髮生蛋白質類%引導骨再生技術
조직공정%신생혈관화%생이성%골형태발생단백질류%인도골재생기술
Tissue engineering%Neovascularization,physiologic%Bone morphogenetic proteins%Guided bone regeneration
目的 探讨引导骨再生(GBR)技术对血管化组织工程骨修复兔股骨缺损过程中局部骨形态发生蛋白-2(BMP-2)的成骨量及表达的影响,以明确GBR技术在血管化组织工程骨应用中的作用. 方法 将兔自体骨髓基质干细胞经诱导后与β-磷酸三钙材料复合,植入制备的兔股骨缺损处并在材料侧槽中植入股动静脉束,其中实验组9例,血管化组织工程骨用可吸收性GBR屏障膜包裹;对照组9例,单纯植入血管化组织工程骨,分别于术后4、8、12周通过形态学检测新生骨量,ELISA法检测骨缺损局部BMP-2的表达量. 结果 随着时间进展各组成骨量逐渐增加(实验组4、8、12周时新生骨的相对面积比分别为7.31%±0.55%,35.23%±3.07%,76.09%±3.71%,对照组4、8、12周时新生骨的相对面积比分别为17.26%±1.17%,54.50%±4.26%,82.57%±4.11%,差异均有统计学意义(P<0.05);且同一时间点实验组成骨量低于对照组,差异有统计学意义(P<0.05);术后4、8、12周时实验组骨缺损局部BMP-2 OD值分别为0.334±0.012,0.245±0.008,0.172±0.009,对照组骨缺损局部BMP-2 OD值分别为0.389±0.008,0.289±0.008,0.189±0.009;术后4周时两组骨缺损内BMP-2表达量均达峰值,此后即开始出现不同程度的下降;术后4、8、12周时骨缺损局部BMP-2表达量实验组均低于对照组,差异均有统计学意义(P<0.05). 结论 GBR屏障膜会降低血管化组织工程骨修复兔股骨缺损局部的成骨量,并减少骨缺损过程中局部BMP-2的表达量.
目的 探討引導骨再生(GBR)技術對血管化組織工程骨脩複兔股骨缺損過程中跼部骨形態髮生蛋白-2(BMP-2)的成骨量及錶達的影響,以明確GBR技術在血管化組織工程骨應用中的作用. 方法 將兔自體骨髓基質榦細胞經誘導後與β-燐痠三鈣材料複閤,植入製備的兔股骨缺損處併在材料側槽中植入股動靜脈束,其中實驗組9例,血管化組織工程骨用可吸收性GBR屏障膜包裹;對照組9例,單純植入血管化組織工程骨,分彆于術後4、8、12週通過形態學檢測新生骨量,ELISA法檢測骨缺損跼部BMP-2的錶達量. 結果 隨著時間進展各組成骨量逐漸增加(實驗組4、8、12週時新生骨的相對麵積比分彆為7.31%±0.55%,35.23%±3.07%,76.09%±3.71%,對照組4、8、12週時新生骨的相對麵積比分彆為17.26%±1.17%,54.50%±4.26%,82.57%±4.11%,差異均有統計學意義(P<0.05);且同一時間點實驗組成骨量低于對照組,差異有統計學意義(P<0.05);術後4、8、12週時實驗組骨缺損跼部BMP-2 OD值分彆為0.334±0.012,0.245±0.008,0.172±0.009,對照組骨缺損跼部BMP-2 OD值分彆為0.389±0.008,0.289±0.008,0.189±0.009;術後4週時兩組骨缺損內BMP-2錶達量均達峰值,此後即開始齣現不同程度的下降;術後4、8、12週時骨缺損跼部BMP-2錶達量實驗組均低于對照組,差異均有統計學意義(P<0.05). 結論 GBR屏障膜會降低血管化組織工程骨脩複兔股骨缺損跼部的成骨量,併減少骨缺損過程中跼部BMP-2的錶達量.
목적 탐토인도골재생(GBR)기술대혈관화조직공정골수복토고골결손과정중국부골형태발생단백-2(BMP-2)적성골량급표체적영향,이명학GBR기술재혈관화조직공정골응용중적작용. 방법 장토자체골수기질간세포경유도후여β-린산삼개재료복합,식입제비적토고골결손처병재재료측조중식입고동정맥속,기중실험조9례,혈관화조직공정골용가흡수성GBR병장막포과;대조조9례,단순식입혈관화조직공정골,분별우술후4、8、12주통과형태학검측신생골량,ELISA법검측골결손국부BMP-2적표체량. 결과 수착시간진전각조성골량축점증가(실험조4、8、12주시신생골적상대면적비분별위7.31%±0.55%,35.23%±3.07%,76.09%±3.71%,대조조4、8、12주시신생골적상대면적비분별위17.26%±1.17%,54.50%±4.26%,82.57%±4.11%,차이균유통계학의의(P<0.05);차동일시간점실험조성골량저우대조조,차이유통계학의의(P<0.05);술후4、8、12주시실험조골결손국부BMP-2 OD치분별위0.334±0.012,0.245±0.008,0.172±0.009,대조조골결손국부BMP-2 OD치분별위0.389±0.008,0.289±0.008,0.189±0.009;술후4주시량조골결손내BMP-2표체량균체봉치,차후즉개시출현불동정도적하강;술후4、8、12주시골결손국부BMP-2표체량실험조균저우대조조,차이균유통계학의의(P<0.05). 결론 GBR병장막회강저혈관화조직공정골수복토고골결손국부적성골량,병감소골결손과정중국부BMP-2적표체량.
Objective To evaluate the effect of guided bone regeneration (GBR) on expression level of bone morphogenetic protein-2 (BMP-2) and bone formation in repair of femoral defects with vascularized engineered-bone in rabbits. Methods Eighteen healthy New Zealand rabbits were randomized into 2 e-qual groups. A segmental bone defect of 150 mm in length was made at the right femur in each rabbit and fixed with a plate. The gaps in the experiment group were plugged with the engineered bones and femoral vascular bundle, which were wrapped by GBR membrane. The vascularized engineered-bone plugged in the gaps in the control group, however, was not wrapped by GBR membrane. The expression levels of BMP-2 of the implants were examined by ELISA kits 4, 8, 12 weeks after implantation. Results The new bone formation was significantly higher in the control group at the end of 4, 8, 12 weeks (P < 0.05). The expression levels of BMP-2 in the control group were also siguificantly higher than in the experimental group at all time points after operation (P<0.05). The expression of BMP-2 peaked at 4 weeks. Conclusion GBR will down-regulate BMP-2 release in models of femoral defect in rabbits.
