中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2008年
4期
16-18
,共3页
吴和顺%曾昭孟%陈树添%郑则钦%郑会丰
吳和順%曾昭孟%陳樹添%鄭則欽%鄭會豐
오화순%증소맹%진수첨%정칙흠%정회봉
大肠癌%DPC4%表达缺失%免疫组化
大腸癌%DPC4%錶達缺失%免疫組化
대장암%DPC4%표체결실%면역조화
Colorectal carcinoma%Deletedin pancreatic caneerlocus4%Absence of expression%Immuno- histotogical methods
目的 探讨抑癌基因DPC4(deletedinpancreaticcancerlocus4)蛋白在大肠癌组织中的缺失表达特征及临床意义,阐明DPC4蛋白缺失表达与大肠癌发生的关系.方法 采用免疫组化(SP法)检测50例大肠癌组织、癌旁组织及正常大肠组织中DPC4的表达,以20例正常大肠组织作为对照分析.结果 50例大肠癌组织中DPC4阳性率为32%,癌旁组织中DPC4阳性率为34%,正常大肠组织DPC4阳性率100%,癌和癌旁的DPC4表达与正常大肠组织DPC4表达的差异有统计学意义(P<0.01).大肠癌组织中DPC4蛋白表达随着病理组织学分化程度下降而降低,淋巴结转移组DPC4的阳性表达低于淋巴结无转移组,但差异无统计学意义.结论 DPC4蛋白表达缺失是大肠癌的发生发展的重要因素之一.
目的 探討抑癌基因DPC4(deletedinpancreaticcancerlocus4)蛋白在大腸癌組織中的缺失錶達特徵及臨床意義,闡明DPC4蛋白缺失錶達與大腸癌髮生的關繫.方法 採用免疫組化(SP法)檢測50例大腸癌組織、癌徬組織及正常大腸組織中DPC4的錶達,以20例正常大腸組織作為對照分析.結果 50例大腸癌組織中DPC4暘性率為32%,癌徬組織中DPC4暘性率為34%,正常大腸組織DPC4暘性率100%,癌和癌徬的DPC4錶達與正常大腸組織DPC4錶達的差異有統計學意義(P<0.01).大腸癌組織中DPC4蛋白錶達隨著病理組織學分化程度下降而降低,淋巴結轉移組DPC4的暘性錶達低于淋巴結無轉移組,但差異無統計學意義.結論 DPC4蛋白錶達缺失是大腸癌的髮生髮展的重要因素之一.
목적 탐토억암기인DPC4(deletedinpancreaticcancerlocus4)단백재대장암조직중적결실표체특정급림상의의,천명DPC4단백결실표체여대장암발생적관계.방법 채용면역조화(SP법)검측50례대장암조직、암방조직급정상대장조직중DPC4적표체,이20례정상대장조직작위대조분석.결과 50례대장암조직중DPC4양성솔위32%,암방조직중DPC4양성솔위34%,정상대장조직DPC4양성솔100%,암화암방적DPC4표체여정상대장조직DPC4표체적차이유통계학의의(P<0.01).대장암조직중DPC4단백표체수착병리조직학분화정도하강이강저,림파결전이조DPC4적양성표체저우림파결무전이조,단차이무통계학의의.결론 DPC4단백표체결실시대장암적발생발전적중요인소지일.
Objective To study the clinical significance of absence of expression of anti-oncogene DPC4 in tissue of colorectal carcinoma,and to reveal the relationship between absence expression of DPC4 and development of eolorectal carcinoma. Methods The expression of DPC4 was detected by SP immuno-histotogi-cal methods in tissue sample from 50 cases of colorectal carcinoma,20 cases of normal tissue of large intestine was treated as control. Results The expression of DPC4 in tissue of co]orectal carcinoma was 32% ,lateral tis-sue 34% ,normal tissue 100%. The expression of DPC4 in tissue and lateral tissue of colorectal carcinoma was significant lower then normal tissue (P<0.01). The expression of DPC4 in tissue and lateral tissue of colorectal carcinoma decreased according to the descending of differentiation of pathologic histology and the transfusion of lymphaden. Conclusion The absence of DPC4 was one of the important factor in the development of colorectalcarcinoma.
