CAJ | 학술논문

目的从人源性噬菌体抗体库中筛选人抗HBsAg Fab噬菌体抗体。方法用固相化HBsAg对所构建的噬菌体抗体库进行三轮淘筛，从中筛选人抗HBsAg Fab噬菌体抗体。结果第三轮淘筛后洗脱下来的噬菌体，较第一轮增加80倍，含有抗体轻链基因和重链基因的克隆，也由淘筛前的17%增至100%，经ELISA证实，筛选到的抗HBsAg噬菌体抗体对HBsAg具有良好的结合活性，而与牛血清白蛋白和HAVAg等无关抗原均不反应。结论 HBsAg对抗体库的淘筛，富集了表面呈现抗HBsAg的单克隆抗体的噬菌体。
목적종인원성서균체항체고중사선인항HBsAg Fab서균체항체。방법용고상화HBsAg대소구건적서균체항체고진행삼륜도사，종중사선인항HBsAg Fab서균체항체。결과제삼륜도사후세탈하래적서균체，교제일륜증가80배，함유항체경련기인화중련기인적극륭，야유도사전적17%증지100%，경ELISA증실，사선도적항HBsAg서균체항체대HBsAg구유량호적결합활성，이여우혈청백단백화HAVAg등무관항원균불반응。결론 HBsAg대항체고적도사，부집료표면정현항HBsAg적단극륭항체적서균체。
Objective Selecting Fab phage antibodies to HBsAg from the phage antibody library. Methods The phages displaying antibody fragments were subjected to three rounds of panning with HBsAg coated in solid phase.Results The eluted phages were enriched nearly 80-fold,and the percentage of recombinant clones increased from 17% to 100% after the third round of panning. The HBsAg specific monoclonal antibodies were determined by ELISA. These antibodies combined specifically with HBsAg, but not with BSA and HAV antigens. Conclusion Our work on constructing human immunoglobulin combinatorial library and selecting anti-HBsAg Fab phage antibodies would be beneficial to the further study on the production of soluble Fab antibodies.