CAJ | 학술논문

红色糖多孢菌是一种工业上规模化生产红霉素的放线菌。针对氮代谢中的重要转录调控因子GlnR展开相关功能研究,了解其作用机制。通过大肠杆菌克隆表达系统体外表达得到了GlnR蛋白,并用ESMA试验分析验证GlnR对amt-glnB和glnA1具有调控作用。这为探索红色糖多孢菌氮代谢调控机制和红霉素合成调控机制提供了一个新的突破点。
홍색당다포균시일충공업상규모화생산홍매소적방선균。침대담대사중적중요전록조공인자GlnR전개상관공능연구,료해기작용궤제。통과대장간균극륭표체계통체외표체득도료GlnR단백,병용ESMA시험분석험증GlnR대amt-glnB화glnA1구유조공작용。저위탐색홍색당다포균담대사조공궤제화홍매소합성조공궤제제공료일개신적돌파점。
Saccharopolyspora erythraea is used for the industrial-scale production of the antibiotic erythromycin A,derivatives of which play a vital role in medicine.Focused on the transcription factor GlnR involved in nitrogen metabolism and related fuction,the mechanism was studied.GlnR protein was obtained by E.coli expression system,and GlnR was found to regulate amt-glnB and glnA1 involved in nitrogen metabolism using EMSA.The research provided a new breakthrough to explore the nitrogen metabolism and erythromycin biosynthesis mechanism in S.erythraea.