CAJ | 학술논문

目的：探讨Bcl-xl、p53基因在条斑紫菜多糖抑制BGC-823细胞增殖和诱导凋亡中的作用。方法：将不同浓度条斑紫菜多糖作用于BGC-823细胞,利用MTT法检测细胞增殖抑制率,RT-PCR法检测Bcl-xl、p53基因表达量的变化、流式细胞仪检测细胞周期和细胞凋亡情况。结果：条斑紫菜多糖能抑制BGC-823细胞增殖及诱导细胞凋亡,并上调p53和降低Bcl-xl表达。结论：条斑紫菜多糖抑制人胃癌细胞增殖、促进凋亡,其机制可能与激活p53、抑制Bcl-xl表达有关。
목적：탐토Bcl-xl、p53기인재조반자채다당억제BGC-823세포증식화유도조망중적작용。방법：장불동농도조반자채다당작용우BGC-823세포,이용MTT법검측세포증식억제솔,RT-PCR법검측Bcl-xl、p53기인표체량적변화、류식세포의검측세포주기화세포조망정황。결과：조반자채다당능억제BGC-823세포증식급유도세포조망,병상조p53화강저Bcl-xl표체。결론：조반자채다당억제인위암세포증식、촉진조망,기궤제가능여격활p53、억제Bcl-xl표체유관。
【Objective】To investigate the effect of Bcl-xl and p53 gene on BGC-823 cells proliferation and apoptosis induced by Porphyra yezoensis polysaccharide.【Method】 BGC-823 cells were treated with various of Porphyra yezoensis polysaccharide.We detected the rate of cell proliferation inhibition by MTT assay and the levels of Bcl-xl and p53 gene expression by RT-PCR.We detected cell cycle and cell apoptosis by flow cytometry.【Result】Porphyra yezoensis polysaccharide could effectively inhibit the BGC-823 cells proliferation and induce apoptosis,and it could effectively decrease the level of Bcl-xl gene expression and recrease the level of p53 gene expression.【Conclusion】Porphyra yezoensis polysaccharide could inhibite the BGC-823 cells proliferation and induce apoptosis of BGC-823 cells probably through down regulating Bcl-xl gene expression and up regulating p53 gene expression.