CAJ | 학술논문

检测了番木瓜果实3个发育时期（Ⅰ、Ⅱ、Ⅲ）的果皮（外果皮）、果肉（内果皮）及种子中芥子酶的活性,并通过RT-PCR分析CpTGG1、CpTGG2和CpTGG3三个番木瓜芥子酶基因在番木瓜果实各组织的差异性表达情况。结果表明,番木瓜果皮及种子中均有明显的芥子酶活性,但在果肉中无法检测出芥子酶活性;成熟后（Ⅲ期）番木瓜果皮的芥子酶活性相比未成熟前提高了6.6倍,而不同发育时期种子中的芥子酶并不表现出显著差异性。RT-PCR分析结果表明,番木瓜芥子酶基因在番木瓜果实中的表达存在显著时空差异性。其中,CpTGG1在番木瓜果皮及种子中均有表达,且表达量无明显差异,在果肉中完全无表达;CpTGG2在番木瓜果实的各个组织中均无表达;CpTGG3在果皮及种子中也均有表达,成熟果皮（Ⅲ期）和Ⅰ期种子中表达量比较强,并随着果实的发育成熟而呈现梯度变化。
검측료번목과과실3개발육시기（Ⅰ、Ⅱ、Ⅲ）적과피（외과피）、과육（내과피）급충자중개자매적활성,병통과RT-PCR분석CpTGG1、CpTGG2화CpTGG3삼개번목과개자매기인재번목과과실각조직적차이성표체정황。결과표명,번목과과피급충자중균유명현적개자매활성,단재과육중무법검측출개자매활성;성숙후（Ⅲ기）번목과과피적개자매활성상비미성숙전제고료6.6배,이불동발육시기충자중적개자매병불표현출현저차이성。RT-PCR분석결과표명,번목과개자매기인재번목과과실중적표체존재현저시공차이성。기중,CpTGG1재번목과과피급충자중균유표체,차표체량무명현차이,재과육중완전무표체;CpTGG2재번목과과실적각개조직중균무표체;CpTGG3재과피급충자중야균유표체,성숙과피（Ⅲ기）화Ⅰ기충자중표체량비교강,병수착과실적발육성숙이정현제도변화。
Using the method of myrosinase activity detecting and reverse transcription-polymerase chain reaction（RT-PCR） analysis,we took the peel,pulp and seeds into study,which from three different development stage papaya fruit.The result showed that there was significantly myrosinase activity found in peel and seeds,no myrosinase activity in pulp,and the myrosinase activity in the peel of ripening fruit increase in amounts of 6.6 times than the green one.RT-PCR analysis revealed that CpTGG1 was expressed in peel and seeds differ little from different development stage;CpTGG2 was turned off in all the tissues in papaya fruit of any stage;while CpTGG3 was expressed in peel and seeds with a gradual change,suggesting that myrosinase genes in papaya are spatially regulated.