CAJ | 학술논문

采用限制性内切酶Sau3AI、MspI、TaqαI、AluI分别对获得的L16基因序列进行理论酶切。通过限制性内切酶酶切后,酶切片段大小相同的记为1,不同的记为0。相似系数和聚类分析应用NTSYS-pc2.01数据分析软件进行,采用Nei的方法计算相似系数,UPGMA方法聚类,绘制树状图。结果表明芭蕉属种间存在较为丰富的cpDNA PCR-RFLP多态性,种内差异性不明显,有些亚种间无法区分开。而M.acuminata具有丰富的亚种和变种,遗传差异大,多样性丰富。
채용한제성내절매Sau3AI、MspI、TaqαI、AluI분별대획득적L16기인서렬진행이론매절。통과한제성내절매매절후,매절편단대소상동적기위1,불동적기위0。상사계수화취류분석응용NTSYS-pc2.01수거분석연건진행,채용Nei적방법계산상사계수,UPGMA방법취류,회제수상도。결과표명파초속충간존재교위봉부적cpDNA PCR-RFLP다태성,충내차이성불명현,유사아충간무법구분개。이M.acuminata구유봉부적아충화변충,유전차이대,다양성봉부。
In this research,four restriction enzymes were used,which are Sau3AI,MspI,TaqαI and AluI,to slice to the L16 sequeces by theory enzymes slice method.After slice,the same or alike dimensions were recorded to 1,dissimilarities were recorded to 0.Alike coefficient and gather a type analytical were analyzed by NTSYS-pc 2.01 data analysis software progress,adopt Nei method computing alike coefficient,the UPGMA method gathers a type and then draws tree diagram.The result suggests that the Musa have a colourful cpDNA PCR-RFLP genetic diversity between kinds.But there is not obvious genetic diversity,and some subspecies can not differentiate.In addition,M.acuminata has prolific subspecies and aberrant species,and have a big heredity discrepancy.