青海畜牧兽医杂志
青海畜牧獸醫雜誌
청해축목수의잡지
CHINESE QINGHAI JOURNAL OF ANIMAL AND VETERINARY SCIENCES
2011年
6期
11-12
,共2页
胡勇%靳义超%吴海玥%仇桂芳%韩银仓%冶成君
鬍勇%靳義超%吳海玥%仇桂芳%韓銀倉%冶成君
호용%근의초%오해모%구계방%한은창%야성군
藏羊血%血红素%提取工艺
藏羊血%血紅素%提取工藝
장양혈%혈홍소%제취공예
Tibetan sheep blood%hemoglobin%extraction technology
通过正交试验确定了藏羊血中提取血红素的最优工艺条件,即:溶血时添加蒸馏水1倍、氯仿5倍、分离血红素时加丙酮5倍、离心时间为20min,pH值为3。
通過正交試驗確定瞭藏羊血中提取血紅素的最優工藝條件,即:溶血時添加蒸餾水1倍、氯倣5倍、分離血紅素時加丙酮5倍、離心時間為20min,pH值為3。
통과정교시험학정료장양혈중제취혈홍소적최우공예조건,즉:용혈시첨가증류수1배、록방5배、분리혈홍소시가병동5배、리심시간위20min,pH치위3。
Extraction technology of hemoglobin from Tibetan sheep blood was studied and the optimal process conditions were made by orthogonal expts.That is: To add the same volume distilled water and five times chloroform as the blood is dissilved.five times of acetone is added when blood cells is isolated.Meanwhile adjust the pH to 3 and then centrifuge it for 20 minuts.