农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2011年
8期
1116-1117,1124
,共3页
朱艳芳%毛春娜%张爱民%薛建平%李佳娣%宋运贤
硃豔芳%毛春娜%張愛民%薛建平%李佳娣%宋運賢
주염방%모춘나%장애민%설건평%리가제%송운현
半夏%叶柄%疏松愈伤组织%悬浮培养
半夏%葉柄%疏鬆愈傷組織%懸浮培養
반하%협병%소송유상조직%현부배양
Pinellia ternata%Petiole%Loose callus%Suspension culture
[目的]建立半夏细胞悬浮培养体系。[方法]以半夏叶柄为外植体,采用正交试验设计研究了2,4-D、NAA、毒莠定和KT4种植物生长物质及其配比对疏松愈伤组织形成的影响。[结果]2,4-D和毒莠定对半夏叶柄愈伤组织诱导效果最显著,KT和NAA次之。诱导半夏叶柄形成疏松愈伤组织的适宜培养基为MS+0.5mg/L2,4-D+1.0mg/LNAA+1.0mg/L毒莠定+1.5mg/LKT。[结论]为通过半夏细胞悬浮提取有效成分和生产人工种子奠定了基础。
[目的]建立半夏細胞懸浮培養體繫。[方法]以半夏葉柄為外植體,採用正交試驗設計研究瞭2,4-D、NAA、毒莠定和KT4種植物生長物質及其配比對疏鬆愈傷組織形成的影響。[結果]2,4-D和毒莠定對半夏葉柄愈傷組織誘導效果最顯著,KT和NAA次之。誘導半夏葉柄形成疏鬆愈傷組織的適宜培養基為MS+0.5mg/L2,4-D+1.0mg/LNAA+1.0mg/L毒莠定+1.5mg/LKT。[結論]為通過半夏細胞懸浮提取有效成分和生產人工種子奠定瞭基礎。
[목적]건립반하세포현부배양체계。[방법]이반하협병위외식체,채용정교시험설계연구료2,4-D、NAA、독유정화KT4충식물생장물질급기배비대소송유상조직형성적영향。[결과]2,4-D화독유정대반하협병유상조직유도효과최현저,KT화NAA차지。유도반하협병형성소송유상조직적괄의배양기위MS+0.5mg/L2,4-D+1.0mg/LNAA+1.0mg/L독유정+1.5mg/LKT。[결론]위통과반하세포현부제취유효성분화생산인공충자전정료기출。
[Objective] The research aimed to establish the cell suspension culture system of Pinellia ternate.[Method] The petiole of Pinellia ternate was as the explant.The orthogonal test was used to study the influences of four plant growth substances(2,4-D,NAA,picloram and KT)and their mixture ratios on the formation of loose callus.[Result] The induction effect of 2,4-D and picloram on the petiole callus of Pinellia ternate was the most significant.Then,the second ones were KT and NAA.The optimal medium which induced Pinellia ternate petiole to form the loose callus was MS+0.5 mg/L of 2,4-D+1.0 mg/L of NAA+1.0 mg/L of picloram+1.5 mg/L of KT.[Conclusion] The research laid the foundation for extracting the active ingredient from the cell suspension of Pinellia ternate and producing the artificial seed.