基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
Genomics and Applied Biology
2011年
4期
265-272
,共8页
水稻%尿苷二磷酸葡萄糖焦磷酸化酶%OsUgp2%otsAB%共表达
水稻%尿苷二燐痠葡萄糖焦燐痠化酶%OsUgp2%otsAB%共錶達
수도%뇨감이린산포도당초린산화매%OsUgp2%otsAB%공표체
Rice%UDP-glucose pyrophosphorylase （UGPase）%Os Ugp2%otsA B%Co-expression
本研究通过农杆菌介导的转化技术，将水稻尿苷-2磷酸葡萄糖焦磷酸化酶基N（OsUgp2）与大肠杆菌海藻糖-6-磷酸合成酶／海藻糖-6-磷酸合成酶磷脂酶融合基因（otsAB）共表达载体转化水稻愈伤组织，对获得的转基因植株分别进行干旱和寒冷处理，试图明确0sUgp2和otsAB共表达是否可以进一步提高转基因水稻植株的抗胁迫能力。表型观察结果显示0sUgp2和。拈AB共表达植株（ABu）经干旱和寒冷处理后恢复生长的能力，较otsAB和OsUgp2分别过量表达的转化植株（AB和U）均有所提高。定量PCR检测结果显示：经干旱处理后，转化植株ABU中0tsAB和OsUgp2的表达量较未处理植株分别提高1．92倍和1．67倍，与转化植株AB和I中0tsAB和OsUgp2表达的增加量相近；而寒冷处理后，转化植株ABU中otsAB和OsUgp2的表达量分别增加2．68倍和2．04倍，明显高于转化植株AB和u中otsAB和OsUgp2表达的增加量。液相色谱检测结果显示：OsUgp2和otsAB共表达和单表达均可以提高转化植株中海藻糖的生物合成量，且共转化植株ABU中海藻糖的含量在干旱和寒冷处理前后均是最高的。干旱和寒冷处理后，共转化植株ABU中蔗糖含量的增加也较明显。本研究结果表明OsUgp2和otsAB共表达可以增加转基因水稻植株中海藻糖和蔗糖分子的含量，同时转基因水稻植株的抗旱和耐冷胁迫的能力也得到了一定提高。
本研究通過農桿菌介導的轉化技術，將水稻尿苷-2燐痠葡萄糖焦燐痠化酶基N（OsUgp2）與大腸桿菌海藻糖-6-燐痠閤成酶／海藻糖-6-燐痠閤成酶燐脂酶融閤基因（otsAB）共錶達載體轉化水稻愈傷組織，對穫得的轉基因植株分彆進行榦旱和寒冷處理，試圖明確0sUgp2和otsAB共錶達是否可以進一步提高轉基因水稻植株的抗脅迫能力。錶型觀察結果顯示0sUgp2和。拈AB共錶達植株（ABu）經榦旱和寒冷處理後恢複生長的能力，較otsAB和OsUgp2分彆過量錶達的轉化植株（AB和U）均有所提高。定量PCR檢測結果顯示：經榦旱處理後，轉化植株ABU中0tsAB和OsUgp2的錶達量較未處理植株分彆提高1．92倍和1．67倍，與轉化植株AB和I中0tsAB和OsUgp2錶達的增加量相近；而寒冷處理後，轉化植株ABU中otsAB和OsUgp2的錶達量分彆增加2．68倍和2．04倍，明顯高于轉化植株AB和u中otsAB和OsUgp2錶達的增加量。液相色譜檢測結果顯示：OsUgp2和otsAB共錶達和單錶達均可以提高轉化植株中海藻糖的生物閤成量，且共轉化植株ABU中海藻糖的含量在榦旱和寒冷處理前後均是最高的。榦旱和寒冷處理後，共轉化植株ABU中蔗糖含量的增加也較明顯。本研究結果錶明OsUgp2和otsAB共錶達可以增加轉基因水稻植株中海藻糖和蔗糖分子的含量，同時轉基因水稻植株的抗旱和耐冷脅迫的能力也得到瞭一定提高。
본연구통과농간균개도적전화기술，장수도뇨감-2린산포도당초린산화매기N（OsUgp2）여대장간균해조당-6-린산합성매／해조당-6-린산합성매린지매융합기인（otsAB）공표체재체전화수도유상조직，대획득적전기인식주분별진행간한화한랭처리，시도명학0sUgp2화otsAB공표체시부가이진일보제고전기인수도식주적항협박능력。표형관찰결과현시0sUgp2화。념AB공표체식주（ABu）경간한화한랭처리후회복생장적능력，교otsAB화OsUgp2분별과량표체적전화식주（AB화U）균유소제고。정량PCR검측결과현시：경간한처리후，전화식주ABU중0tsAB화OsUgp2적표체량교미처리식주분별제고1．92배화1．67배，여전화식주AB화I중0tsAB화OsUgp2표체적증가량상근；이한랭처리후，전화식주ABU중otsAB화OsUgp2적표체량분별증가2．68배화2．04배，명현고우전화식주AB화u중otsAB화OsUgp2표체적증가량。액상색보검측결과현시：OsUgp2화otsAB공표체화단표체균가이제고전화식주중해조당적생물합성량，차공전화식주ABU중해조당적함량재간한화한랭처리전후균시최고적。간한화한랭처리후，공전화식주ABU중자당함량적증가야교명현。본연구결과표명OsUgp2화otsAB공표체가이증가전기인수도식주중해조당화자당분자적함량，동시전기인수도식주적항한화내랭협박적능력야득도료일정제고。
Rice UDP-glucose pyrophosphorylase gene （Os Ugp2） and a fusion gene oftrehalose-6-phosphate synthase/trehalose-6-phosphate phosphatase （otsA B） in E. coli were co-expressed in rice in order to find out if the ability of drought and cold resistance in transgenic rice will be further improved. The phenotypes of the drought and cold treated rice seedlings showed that the surviving ability of transgenic rice co-expressed OsUgp2 and otsA B （ABU） was better than that of rice over-expressed otsAB （AB） or Os Ugp2 （U） separately. The results of realtime PCR revealed that the expression levels of otsAB and OsUgp2 in ABU were increased 1.92 and 1.67-folds after drought treatment, which was similar with that in AB and U. However, the expression quantities of otsA B and Os- Ugp2 in ABU were increased 2.68 and 2.04-folds after cold treatment, which was much higher than that in AB and U. Furthermore, the detection of liquid chromatography indicated that the contents of trehalose in ABU, AB and U were all increased compared with non-transgenic rice ZH11, and that the content oftrehalose in ABU remained the highest preor after drought and cold treatments among the three. Besides, the content of sucrose in ABU was also found enhanced eminently after drought and cold treatments. It was concluded that co-expression of Os Ugp2 and otsA B led to the increase in the contents oftrehalose and sucrose in transgenic rice, which were considered correlative to its improved resistance to drought and cold stresses.
