CAJ | 학술논문

在蓝藻水华的形成因素中,氮磷比例是一个很大的影响因素,其最适N/P为16∶1。因此笔者设想,该N/P值是与细胞的生长分裂相关系的。磷在细胞中的主要作用与细胞DNA、RNA的合成有关;氮则是参与了蛋白质的合成,氮磷之间合适的比例,就代表了细胞生长与分裂之间量的关系。为了验证该设想,通过流式细胞术P I染色法来检测微囊藻细胞的DNA含量,设置不同的N/P比值,缺NP、1∶1、16∶1、160∶1四组,结果表明在N/P为16∶1时,藻细胞的DNA含量较其他对照组高,间接地反映了藻细胞的数目,即当N/P为16∶1藻细胞处于分裂的最佳水平。
재람조수화적형성인소중,담린비례시일개흔대적영향인소,기최괄N/P위16∶1。인차필자설상,해N/P치시여세포적생장분렬상관계적。린재세포중적주요작용여세포DNA、RNA적합성유관;담칙시삼여료단백질적합성,담린지간합괄적비례,취대표료세포생장여분렬지간량적관계。위료험증해설상,통과류식세포술P I염색법래검측미낭조세포적DNA함량,설치불동적N/P비치,결NP、1∶1、16∶1、160∶1사조,결과표명재N/P위16∶1시,조세포적DNA함량교기타대조조고,간접지반영료조세포적수목,즉당N/P위16∶1조세포처우분렬적최가수평。
In the formation of algae bloom,the proportion of nitrogen and phosphorus is the major influential factor.The optimal N/P for alage is 16：1.It is assumed that the particular proportion be related to the growth and division of cyanobacteria cells.The most vital role in vivo of phosphorus is involved in DNA、RNA synthesis.As for nitrogen,it is related to the protein synthesis.The appropriate ratio between N and P may be presumed as a reflection of the relation in quantity between the growth and division of alage cell.In order to verify the assumption,we used the technology of FCM to detect the cells of M.aeruginosa stained by PI which were cultured on different N/P nutrients and set up four groups of different N/P ratios,16∶1,160∶1,1∶1 and lacking of NP.It was found that when N/P=16∶1,the DNA content of M.aeruginosa cells is higher than others.We concluded that the division of these cells is at an optimal level.