CAJ | 학술논문

为了弄清理化因素及作用方式对霉菌的影响,采用滤纸片法与挥发性物质活性检测法,研究不同浓度柠檬醛对绳状青霉(Penicillium funiculosum)抑制作用,并检测不同处理时间对孢子萌发率的影响.结果表明,柠檬醛采用滤纸片法与挥发性活性物质检测法对P. funiculosum的最小抑制浓度(MIC)分别为6.0与3.0 mg·mL~(-1).在3.0 mg·mL~(-1)浓度下处理10min孢子萌发受到明显抑制,萌发时间比对照推迟6 h,萌发率降低21.7%;而处理120 min萌发时间比对照延迟10 h,萌发率降低83.8%.柠檬醛可通过熏蒸的方式对P. funiculosum起到较好的抑制作用.这为工农业产品防治霉菌的危害提供了新的理论依据.
위료롱청이화인소급작용방식대매균적영향,채용려지편법여휘발성물질활성검측법,연구불동농도저몽철대승상청매(Penicillium funiculosum)억제작용,병검측불동처리시간대포자맹발솔적영향.결과표명,저몽철채용려지편법여휘발성활성물질검측법대P. funiculosum적최소억제농도(MIC)분별위6.0여3.0 mg·mL~(-1).재3.0 mg·mL~(-1)농도하처리10min포자맹발수도명현억제,맹발시간비대조추지6 h,맹발솔강저21.7%;이처리120 min맹발시간비대조연지10 h,맹발솔강저83.8%.저몽철가통과훈증적방식대P. funiculosum기도교호적억제작용.저위공농업산품방치매균적위해제공료신적이론의거.
Effect of different concentrations of citral on growth inhibition of Penicillium funiculosum were studied by filter paper and volatile activity test method. And the effect of different treated time of citral on percent inhibition of spore germination were studied. Result showed that the minimum inhibitory concentration (MIC) of citral for P. funiculosum was 6.0 mg·mL~(-1) (Filter Paper Method) and 3.0 mg·mL~(-1) (Volatile Active Substances Method), respectively. Significantly inhibition of spore germination was found after it was pretreated by MIC for 10 min. The germination time delayed for 6 h and germination rate reduced by 21.7% than the control. Moreover, when the pretreated time extended to 120 min, sproes germination time delayed for 10 h than the control and the germination rate reduced by 83.8% than the control. Citral exhibited good inhibition for growth and spore germination of P. funiculosum by the fumiga-tion method. Therefore, this paper provided theoretical basis for the use of citral in preventing industrial agricultural products from moulds.