西北植物学报
西北植物學報
서북식물학보
ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA
2010年
1期
120-125
,共6页
羊草%休眠机制%萌发%休眠破除
羊草%休眠機製%萌髮%休眠破除
양초%휴면궤제%맹발%휴면파제
Leymus chinensis%seed dormancy mechanism%germination%seed dormancy breaking
羊草种子休眠程度深、发芽率低是限制栽培利用的重要因子.采用不同破除羊草种子休眠的方法,测定各处理对种子萌发的影响,以探索破除羊草种子休眠的有效途径.结果显示:(1)刺破种皮的裸种子较完整种子的萌发率、吸水速率、生活力分别由对照的6%、63%、0%显著增加到60%、86%、94%.(2)完整羊草种子分别用清水浸种1 d、30% NaOH浸种80 min、清水浸种1 d后用30% NaOH浸种60min其萌发率由6%分别显著提高到36%、60%、84%,而各浓度赤霉素处理完整种子其萌发率较对照均无显著变化. (3)采用清水浸种1 d后用30% NaOH处理60 min,再施加200 μg/g GA3综合处理,可使羊草完整种子的发芽率由6%提高到91%,接近其种子生活力94%.研究表明,羊草种子的稃与种皮不影响种子水分的吸收,但影响种子对GA3的吸收、不同程度地阻碍大分子物质的渗入、限制羊草种子内部萌发抑制物的渗出,从而引起种子休眠;分析认为稃和种皮以及种子内部萌发抑制物质是引起羊草种子休眠的主要原因.
羊草種子休眠程度深、髮芽率低是限製栽培利用的重要因子.採用不同破除羊草種子休眠的方法,測定各處理對種子萌髮的影響,以探索破除羊草種子休眠的有效途徑.結果顯示:(1)刺破種皮的裸種子較完整種子的萌髮率、吸水速率、生活力分彆由對照的6%、63%、0%顯著增加到60%、86%、94%.(2)完整羊草種子分彆用清水浸種1 d、30% NaOH浸種80 min、清水浸種1 d後用30% NaOH浸種60min其萌髮率由6%分彆顯著提高到36%、60%、84%,而各濃度赤黴素處理完整種子其萌髮率較對照均無顯著變化. (3)採用清水浸種1 d後用30% NaOH處理60 min,再施加200 μg/g GA3綜閤處理,可使羊草完整種子的髮芽率由6%提高到91%,接近其種子生活力94%.研究錶明,羊草種子的稃與種皮不影響種子水分的吸收,但影響種子對GA3的吸收、不同程度地阻礙大分子物質的滲入、限製羊草種子內部萌髮抑製物的滲齣,從而引起種子休眠;分析認為稃和種皮以及種子內部萌髮抑製物質是引起羊草種子休眠的主要原因.
양초충자휴면정도심、발아솔저시한제재배이용적중요인자.채용불동파제양초충자휴면적방법,측정각처리대충자맹발적영향,이탐색파제양초충자휴면적유효도경.결과현시:(1)자파충피적라충자교완정충자적맹발솔、흡수속솔、생활력분별유대조적6%、63%、0%현저증가도60%、86%、94%.(2)완정양초충자분별용청수침충1 d、30% NaOH침충80 min、청수침충1 d후용30% NaOH침충60min기맹발솔유6%분별현저제고도36%、60%、84%,이각농도적매소처리완정충자기맹발솔교대조균무현저변화. (3)채용청수침충1 d후용30% NaOH처리60 min,재시가200 μg/g GA3종합처리,가사양초완정충자적발아솔유6%제고도91%,접근기충자생활력94%.연구표명,양초충자적부여충피불영향충자수분적흡수,단영향충자대GA3적흡수、불동정도지조애대분자물질적삼입、한제양초충자내부맹발억제물적삼출,종이인기충자휴면;분석인위부화충피이급충자내부맹발억제물질시인기양초충자휴면적주요원인.
Seed dormancy of Leymus chinensis is one of the most important factors limiting its culture and utilization.Present study investigated the seed dormancy mechanism and dormancy breaking technique of L.chinensis and the results showed that:(1) Piercing naked seed compared with intact seed the germination rate,water absorption rate,viability by contrast,6%,63%,0% significantly increased to 60%,86%,94%;(2)Water soaking one day,30% NaOH soaking 80 min,soaking in 30% NaOH for 60 min after soaking with water for one day could complete germination rate of L.chinensis intact seeds significantly from 6% to 36%,60%,84%,while the different concentration of gibberellin treatment to intact seeds had no significant changes compared with the control;(3) Seeds soaked with 30% NaOH by 60 min after soaked with water one day,and then imposed 200 μg/g GA3,could germinate to 91%,close to the seed viability 94%.This study showed that glume and seed coat did not affect the seed imbibition rate,but affected absorption of GA3 and water absorption had been hampered to varying degrees by macromolecular substances,which led to seed dormancy.These results implied that palea,seed coat,and germination inhibitors contained in the seeds may play an important role in controlling seed dormancy of L.chinensis.
