CAJ | 학술논문

从腐烂朽木及附近的土壤采样,分离到25株具有纤维素分解能力的菌株,其中LY16菌株的纤维素酶活最高.采用形态学观察及分子生物学方法初步鉴定其为里氏木霉(Trichoderma reesei).通过碳氮源优化等试验进行产酶条件研究,得出该菌株最佳的产酶条件,培养基配方为(g/L):微晶纤维素10,麸皮40,蛋白胨4,尿素12,KH_2PO_42,MgSO_4·7H_2O 1,CaCl_2·2H_2O 1,FeSO_4 0.01,MnSO_4 0.004 5,CoCl_2 0.003 6,ZnSO_4 0.0035,培养温度30℃、装液量50mL,转速200 r/min,培养时间为72 h.优化后在该条件下,CMC酶活为202.6 U/mL,FPA酶活最大53.2 U/mL.
종부란후목급부근적토양채양,분리도25주구유섬유소분해능력적균주,기중LY16균주적섬유소매활최고.채용형태학관찰급분자생물학방법초보감정기위리씨목매(Trichoderma reesei).통과탄담원우화등시험진행산매조건연구,득출해균주최가적산매조건,배양기배방위(g/L):미정섬유소10,부피40,단백동4,뇨소12,KH_2PO_42,MgSO_4·7H_2O 1,CaCl_2·2H_2O 1,FeSO_4 0.01,MnSO_4 0.004 5,CoCl_2 0.003 6,ZnSO_4 0.0035,배양온도30℃、장액량50mL,전속200 r/min,배양시간위72 h.우화후재해조건하,CMC매활위202.6 U/mL,FPA매활최대53.2 U/mL.
Samples were collected from rotten wood and soil nearby,and 25 strains that can decompose the cellulose were separated, among which the strain LY16 has the strongest cellulase activity. It was identified as Trichoderma reesei by morphological observation and the molecular biological methods. By carrying out the carbon-nitrogen source and other experiments, we have obtained the optimal conditions in which this strain produces enzyme. The culture compositioon (g/L) contained :avicel 10,wheat bran 40,peptone 4,urea 12,KH_2PO_4 ,MgSO_4 ·7H_2O 1,CaCl_2·2H_2O 1,FeSO_4 0.01,MnSO_4 0.004 5,CoCl_2 0.003 6,ZnSO_4 0.003 5,Culture temperature was 30 ℃ ,Liquid volume in flask was 50 mL, rotational speed was 200 r/min, culture time 72 h. In this condition, the CMCase was 202.6 U/mL,and the FPase was 53.2 U/mL.