CAJ | 학술논문

目的动态检测虎眼万年青总皂苷对二乙基亚硝胺诱发的大鼠肝癌变过程中肝组织中Ki67、血管内皮生长因子受体(Flt-1)表达的影响.方法二乙基亚硝胺诱发大鼠肝癌变,虎眼万年青总皂苷干预,运用免疫组化方法观察并分析第12周末、14周末、18周末肝组织标本中Ki67、Flt-1的表达.结果肝癌变过程中以12周模型组Ki67 LI表达最高,14周及18周模型组Ki67 LI逐渐降低,与12周模型组比较有统计学意义.虎眼万年青总皂苷作用的12周预防组Ki67 LI较低且与相应12周模型组有统计学意义.诱癌14周模型组Flt-1 LI表达最高, 12周及18周模型组Flt-1 LI相对低下,与14周模型组比较有统计学意义.虎眼万年青总皂苷作用的14周预防组、治疗组Flt-1 LI降低,且与模型组有统计学意义.结论二乙基亚硝胺诱发的肝癌变过程中以肝损伤、硬化阶段肝细胞增生活跃,而虎眼万年青总皂苷干预此阶段的肝细胞增生.二乙基亚硝胺诱发的肝癌变过程中在肝癌发生阶段Flt-1表达较高,虎眼万年青总皂苷干预此阶段Flt-1表达.
목적 동태검측호안만년청총조감대이을기아초알유발적대서간암변과정중간조직중Ki67、혈관내피생장인자수체(Flt-1)표체적영향.방법 이을기아초알유발대서간암변,호안만년청총조감간예,운용면역조화방법관찰병분석제12주말、14주말、18주말간조직표본중Ki67、Flt-1적표체.결과 간암변과정중이12주모형조Ki67 LI표체최고,14주급18주모형조Ki67 LI축점강저,여12주모형조비교유통계학의의.호안만년청총조감작용적12주예방조Ki67 LI교저차여상응12주모형조유통계학의의.유암14주모형조Flt-1 LI표체최고, 12주급18주모형조Flt-1 LI상대저하,여14주모형조비교유통계학의의.호안만년청총조감작용적14주예방조、치료조Flt-1 LI강저,차여모형조유통계학의의.결론 이을기아초알유발적간암변과정중이간손상、경화계단간세포증생활약,이호안만년청총조감간예차계단적간세포증생.이을기아초알유발적간암변과정중재간암발생계단Flt-1표체교고,호안만년청총조감간예차계단Flt-1표체.
Objective To study the dynamic expression of Ki67 and Flt-1 in the hepatic tissue in diethylnitrosamine(DEN)_induced hepatocarcinogenesis and the effect interfered with Ornithogalum caudatum Ait total saponin(OCA-TS). Methods Ki67 and Flt-1 were observed with immunohistochemical staining in DEN_induced hepatocarcinogenesis after 12 weeks,14 weeks and 18 weeks respectively.Results The expression of Ki67 labeling index (LI) were significantly higher in the group of experiment than the normal group. The expression of Ki67 LI were the highest in the 12 weeks model group, the OCA-TS interfered the expression of Ki67 LI in this periods significantly. And the Ki67 LI decreased gradually in the 14 weeks and 18 weeks model group, there had significant correlation between the 12 weeks model group respectively. And in these periods the OCA-TS interfered the expression of Ki67 LI not too much. We also found the expression of Flt-1 labeling index (LI) were significantly higher in the group of experiment than the normal group. The expression of Flt-1 LI were the highest in the 14 weeks model group, the OCA-TS interfered the expression of Flt-1 LI in this periods significantly. And the Flt-1 LI decreased in the 12 weeks and 18 weeks model group relatively, there had significant correlation between the 14 weeks model group respectively. And in these periods the OCA-TS interfered the expression of Flt-1 LI not too much.Conclusion The hepatocellular exist hyperplasia in the periods of hepatic trauma and hepatic cirrhosis with DEN induced hepatocarcinogenesis, and the OCA-TS interfered the hepatocellular hyperplasia. The expression of Flt-1 is significantly high in the hepatocellular carcinoma occuring periods, and the OCA-TS interfered the expression of Flt-1 in this periods.