CAJ | 학술논문

目的了解国内猪札如病毒(猪肠道杯状病毒成员)的存在和流行情况.方法从多个腹泻猪场共采集腹泻猪粪便189份,采用RT-PCR方法进行分子流行病学调查.结果检出猪札如病毒阳性病料24份,阳性率为12.70%(24/189).测序后与国外已发表的毒株比较,表明了所测定流行毒株的序列与猪札如病毒代表株Cowden相近(同源性介于75.6%～88.3%之间),证实了其均为猪札如病毒.结论首次发现我国猪群中存在猪札如病毒.
목적 료해국내저찰여병독(저장도배상병독성원)적존재화류행정황.방법 종다개복사저장공채집복사저분편189빈,채용RT-PCR방법진행분자류행병학조사.결과 검출저찰여병독양성병료24빈,양성솔위12.70%(24/189).측서후여국외이발표적독주비교,표명료소측정류행독주적서렬여저찰여병독대표주Cowden상근(동원성개우75.6%～88.3%지간),증실료기균위저찰여병독.결론 수차발현아국저군중존재저찰여병독.
A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.