CAJ | 학술논문

应用微卫星遗传标记对近交系C57BL/6J(B6)小鼠遗传稳定性进行分析.用FAM标记的引物PCR扩增了来自北京和上海三个实验动物生产单位提供的三个B6小鼠群体共15个微卫星位点并进行分型.结果显示,所有位点均处于纯合状态,其中7个位点为多态位点.研究表明各B6群体虽然为高度近变群体,但不同生产单位维持的B6群体之间存在遗传分化.
응용미위성유전표기대근교계C57BL/6J(B6)소서유전은정성진행분석.용FAM표기적인물PCR확증료래자북경화상해삼개실험동물생산단위제공적삼개B6소서군체공15개미위성위점병진행분형.결과현시,소유위점균처우순합상태,기중7개위점위다태위점.연구표명각B6군체수연위고도근변군체,단불동생산단위유지적B6군체지간존재유전분화.
The goal of this study was to investigate the genetic stability of the C57BL/6J (B6) inbred mouse strain maintained in different breeders. Three populations of B6, Popl and Pop2 purchased from Beijing and Pop3 purchased from Shanghai, were examined. Fifteen microsatellite loci reported to be polymorphic among inbred strains were amplified using FAM labeled primers and genotyped with ABI Prism 377 automated sequencer. Seven loci were found polymorphic, and all the loci were homozygous in all the three populations. The present study indicates that genetic variation occurs in different B6 populations although they are still inbred in each breeder. The mechanism of genetic variation is not well understood now, but it is very important to know the precise content of the B6 genome before use of this strain in research,