CAJ | 학술논문

绝经是女性一生中很重要的生理现象之一,它能增加一系列复杂免疫、神经退化、新陈代谢和心血管方面的疾病.血液单核细胞能分化成各种各样的细胞,这些细胞在组织形态发生和免疫应答方面起着很重要的作用.本研究中采用了包含大约14,500 个基因探针的Affymetrix Human U133A 基因芯片来研究健康的绝经前和绝经后女性外周血液单核细胞中的基因表达谱.样本之间的对比分析表明有20个基因上调,20个基因下调.其中的28个基因根据它们的生物过程如细胞繁殖、免疫应答、细胞代谢等等被分成了6个主要的GO类别;剩下的12个基因其生物学功能还没有被鉴定.研究结果支持了我们的假设:血液单核细胞的功能状态确实受到绝经的影响,而且由此带来的改变可能是由全基因组范围的基因表达谱而决定的.本研究中鉴定的一些差异表达基因有可能作为以后研究与绝经相关的系统免疫、神经退化和心血管疾病的候选基因研究.此工作是这个研究方向的第一次尝试,为将来的进一步研究奠定了基础.
절경시녀성일생중흔중요적생리현상지일,타능증가일계렬복잡면역、신경퇴화、신진대사화심혈관방면적질병.혈액단핵세포능분화성각충각양적세포,저사세포재조직형태발생화면역응답방면기착흔중요적작용.본연구중채용료포함대약14,500 개기인탐침적Affymetrix Human U133A 기인심편래연구건강적절경전화절경후녀성외주혈액단핵세포중적기인표체보.양본지간적대비분석표명유20개기인상조,20개기인하조.기중적28개기인근거타문적생물과정여세포번식、면역응답、세포대사등등피분성료6개주요적GO유별;잉하적12개기인기생물학공능환몰유피감정.연구결과지지료아문적가설:혈액단핵세포적공능상태학실수도절경적영향,이차유차대래적개변가능시유전기인조범위적기인표체보이결정적.본연구중감정적일사차이표체기인유가능작위이후연구여절경상관적계통면역、신경퇴화화심혈관질병적후선기인연구.차공작시저개연구방향적제일차상시,위장래적진일보연구전정료기출.
Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune,neurodegenerative,metabolic,and cardiovascular disorders.Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response.We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for ～14,500 genes.Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated.Of these genes,28 were classified into six major GO categories relevant to such biological processes as the cell proliferation,immune response,cellular metabolism,and the others.The remaining 12 genes have yet unidentified biological functions.Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause,and resulting changes may be determined through the genomewide gene expression profiling.Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune,neurodegenerative,and cardiovascular disorders.Our study is only the first attempt in this direction,but it lays a basis for further research.