菌物学报
菌物學報
균물학보
MYCOSYSTEMA
2008年
1期
126-139
,共14页
郑维发%项小燕%陈才法%王尧%赵艳霞%蒋继宏%储成才
鄭維髮%項小燕%陳纔法%王堯%趙豔霞%蔣繼宏%儲成纔
정유발%항소연%진재법%왕요%조염하%장계굉%저성재
medical fungus%carbon source%nitrogen source%treatment of metal ions%sterol accumulation%HMG-CoA reductase activity
Lanosterol and ergosterol are the active principles with potential pharmacological activities in Inonotus obliquus. However, the two sterols are less accumulated in cultured mycelia of the fungus. In this study,different carbon and nitrogen sources and pH levels together with three metal ions were assayed for their effects on accumulation of the two sterols in the fungus. Among the tested media the growth medium consisting of glucose (1.5%), rice powder (0.5%), yeast extract (0.4%), wheat bran (0.1%), KH2PO4 (0.01%) and be increased following the treatment of Ag+, Cu2+ and Ca2+. Supplementing Ag+ at concentrations of 0.28 and 0.35μmol partially inhibited ergosterol biosynthesis, leading to an enhanced accumulation of lanosterol, the presence of intermediates of ergosterol biosynthetic pathway and a reduced accumulation of ergosterol in cultured mycelia of I. obliquus.
