CAJ | 학술논문

基于猪的表达标签数据库电子克隆了猪的GATA-3基因,并通过RT-PCR验证了猪的GATA-3基因的核苷酸序列.测序结果显示猪的GATA-3基因的核苷酸长度由1,760 bp个碱基组成,包括1,335 bp的开放阅读框,编码产物为由444个氨基酸残基组成的多肽.通过半定量RT-PCR检测了GATA-3 mRNA在大白猪的各个组织的表达情况.GATA转录因子家族通常有2个Ⅳ型锌指蛋白结构,根据Ⅳ型锌指蛋白结构序列,使用Mega3.1软件构建了分子进化关系树.系统发育分析表明所有的脊椎动物的GATA转录因子都起源于共同的祖先,拓扑结构也表明进化过程中有多种事件发生,包括基因复制和Ⅳ型锌指蛋白结构域重组,根据所得数据有利于进一步了解GATA家族基因趋同和趋异的进化途径.
기우저적표체표첨수거고전자극륭료저적GATA-3기인,병통과RT-PCR험증료저적GATA-3기인적핵감산서렬.측서결과현시저적GATA-3기인적핵감산장도유1,760 bp개감기조성,포괄1,335 bp적개방열독광,편마산물위유444개안기산잔기조성적다태.통과반정량RT-PCR검측료GATA-3 mRNA재대백저적각개조직적표체정황.GATA전록인자가족통상유2개Ⅳ형자지단백결구,근거Ⅳ형자지단백결구서렬,사용Mega3.1연건구건료분자진화관계수.계통발육분석표명소유적척추동물적GATA전록인자도기원우공동적조선,탁복결구야표명진화과정중유다충사건발생,포괄기인복제화Ⅳ형자지단백결구역중조,근거소득수거유리우진일보료해GATA가족기인추동화추이적진화도경.
Pig GATA-3 cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR),using in silico cloning strategy based on pig dbEST.The length of pig GATA-3 cDNA is 1,760 bp containing a 1,335 bp open reading frame (ORF),which encodes a 444 amino acid protein.Semi-quantitative RT-PCR analysis of GATA-3 mRNA expression was done using the total RNAs from different normal tissues of a large white pig.The GATA-binding family of transcription factors comprised of a subgroup of DNA-binding proteins that both bound the consensus GATA motif and contained the class Ⅳ zinc finger motif.The molecular evolution tree was constructed based on the GATA-3 amino acid sequence and class Ⅳ zinc finger motif using mega 3.1.Phylogeny analysis of GATA factors isolated from vertebrates suggested that the six distinct vertebrate GATAs had descended from a common ancestral sequence,and the topology also suggested multiple modes of evolution including gene duplication and class Ⅳ zinc finger motif recombination.These data helped the authors in illuminating the pathways of divergent and convergent evolution of the GATA-binding family of transcription factors.